DNA Polymerase T4 Enzyme
DNA Polymerase T4
Purity
Molecular Biology Grade
Synonyms
DNA Polymerase T4; DNA Polymerase T4 enzyme
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 20mM potassium phosphate, pH 7.5, 200mM potassium chloride, 2mM DTT, 50% glycerol.
Application Notes
Blunting of DNA ends: fill-in 5'-overhangs or/and removal of 3'-overhangs, see protocol, , Blunting of PCR products with 3'-dA overhangs., , Synthesis of labeled DNA probes by the replacement reaction., , Oligonucleotide-directed site-specific mutagenesis., , Ligation-independent cloning of PCR products.
Quality Control
No conversion of covalently closed circular DNA to nicked DNA was detected after incubation of 10 units of T4 DNA Polymerase with 1ug of pUC19 DNA in 50ul reaction buffer for 4 hours at 37 degree C.
Unit Definition
One unit of T4 DNA Polymerase catalyzes the incorporation of 10nmoles of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37 degree C.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for DNA Polymerase T4 enzyme
Catalyzes 5'=>3' synthesis of DNA. Enzyme has 3'=>5' exonuclease activity but lacks 5'=>3' exonuclease activity. T4 DNA polymerase possessing 3'=>5' exonuclease activity exhibits greater activity on single-stranded DNA than on double-stranded DNA.
Product Categories/Family for DNA Polymerase T4 enzyme
Similar Products
Product Notes
The DNA Polymerase T4 (Catalog #AAA653894) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. Blunting of DNA ends: fill-in 5'-overhangs or/and removal of 3'-overhangs, see protocol,, Blunting of PCR products with 3'-dA overhangs.,, Synthesis of labeled DNA probes by the replacement reaction.,, Oligonucleotide-directed site-specific mutagenesis.,, Ligation-independent cloning of PCR products. Researchers should empirically determine the suitability of the DNA Polymerase T4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Polymerase T4, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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