Creatine Kinase BB Enzyme | Ckb enzyme

Creatine Kinase BB, Human (CKBB)

Cat. Num. AAA653774

• Gene Names: Ckb; Ckbb; Ckbr
• Purity: Purified; Purified
• Synonyms: Creatine Kinase BB; Human (CKBB); Ckb enzyme

• Host: Human brain
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a a liquid in 25mM imidazole, 1mM DTE, pH 6.5, 50% glycerol.

• Application Notes: Specific methodologies have not been tested using this product. Optimal dilutions to be determined by the researcher.
• Activity: ~150Units/ml at 37ºC
• Specific Activity: As reported.
• Unit Definition: One unit will transfer one micromole of phosphate from creatine phosphate to ATP per minute at 37 degree C. Measured at 340nm as one equimolar amount of NADH product by a coupled reaction.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for Ckb enzyme

Molecular Biology; MB-Enzymes; Creatine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 401461784
• NCBI GeneID: 24264
• NCBI Accession #: NP_036661.3
• NCBI GenBank Nucleotide #: NM_012529.3
• UniProt Accession #: P07335; Q499P7; Q5PPJ5; Q6IRE0; Q6P139; A0JPK7
• Molecular Weight: 42,725 Da
• NCBI Official Full Name: creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase, brain
• NCBI Official Symbol: Ckb
• NCBI Official Synonym Symbols: Ckbb; Ckbr
• NCBI Protein Information: creatine kinase B-type; B-CK; brain creatine kinase; creatine kinase B chain
• UniProt Protein Name: Creatine kinase B-type
• Protein Family: Creatine kinase
• UniProt Gene Name: Ckb
• UniProt Synonym Gene Names: Ckbb
• UniProt Entry Name: KCRB_RAT

NCBI Description

brain specific isozyme that catalyzed the interconversion of ATP and creatine to ADP and phosphocreatine; may play a role in ATP generation during cerebellar development [RGD, Feb 2006]

Uniprot Description

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity: ATP + creatine = ADP + phosphocreatine.

Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location: Cytoplasm.

Tissue specificity: In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule. Ref.7

Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.

Sequence caution: The sequence AAH87656.1 differs from that shown. Reason: Erroneous initiation.