Angiotensin Converting Enzyme, Rat Enzyme | Ace enzyme

Angiotensin Converting Enzyme, Rat (ACE)

Cat. Num. AAA653765

• Gene Names: Ace; Dcp1; StsRR92
• Applications: Western Blot, Immunoprecipitation
• Purity: Highly purified (> 95% by SDS-PAGE).
• Synonyms: Angiotensin Converting Enzyme; Rat; Rat (ACE); Ace enzyme

• Host: Rat lung
• Purity/Purification: Highly purified (> 95% by SDS-PAGE).
• Form/Format: Supplied as a liquid in 100mM PBS, 150mM sodium chloride, pH 7.4 with 0.2mM CHAPS. No preservatives added.

• Applicable Applications for Ace enzyme: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use as a reference in SDS-PAGE and Western blotting (together with anti-ACE monoclonal), as a reference antigen for immunoprecipitation, and as a source of pure ACE for different enzymatic studies of ACE.; Dilution: Western Blot: 500ng/lane if gel is developed with anti-ACE antibody. Optimal dilutions to be determined by the researcher.
• Activity: ~7mU/ ug of protein; (One unit will produce 1uMole of hippuric acid or His-Leu from Z-Phe-His-Leu per minute in 0.1M phosphate buffer, 30 mM sodium chloride, at pH 8.3 at 37 degree C).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Ace enzyme

ACE (kininase II, EC 3.4.15.1) is a zinc dipeptidyl carboxypeptidase. ACE catalyses both angiotensin I transformation to angiotensis II (a potent vasopressor agent) and degradation of bradikinin, a vaso-depressor. ACE appears to play a key role in regulating vascular tone and remodeling the development of atherosclerotic lesions. Increasing evidence suggests that ACE plays an important role in vascular pathology. Recent studies shows two new functions of this important enzyme: 1) ACE enhances the presentation of endogenous antigens (in particularly HIV 1 gp160-derived peptide p18) to MHC class I-restricted T-lynphocytes (ref. 1); 2) amino-terminal domain of ACE cleaves effectively hemoregulatory tetrapeptide N-Ac-Ser-Asp-Lys-Pro (ref. 2,3) which is involved in the control of hemapoetic stem cell proliferation (the amount of this peptide greatly decreases in patients undergoing cancer chemotherapy) (ref. 4).

Product Categories/Family for Ace enzyme

Molecular Biology; MB-Enzymes; Angiotensin Converting (ACE)

NCBI and Uniprot Product Information

• NCBI GI #: 6978757
• NCBI GeneID: 24310
• NCBI Accession #: NP_036676.1
• NCBI GenBank Nucleotide #: NM_012544.1
• UniProt Accession #: P47820; Q7TMC6; Q8CFN1; Q9EQM9
• Molecular Weight: 150,908 Da
• NCBI Official Full Name: angiotensin-converting enzyme
• NCBI Official Synonym Full Names: angiotensin I converting enzyme
• NCBI Official Symbol: Ace
• NCBI Official Synonym Symbols: Dcp1; StsRR92
• NCBI Protein Information: angiotensin-converting enzyme; kininase II; dipeptidyl carboxypeptidase I; angiotensin 1 converting enzyme 1; angiotensin I converting enzyme 1; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; Dipeptidyl carboxypeptidase 1 (Angiotensin I-converting enzyme); angiotensin I-converting enzyme (Dipeptidyl carboxypeptidase 1)
• UniProt Protein Name: Angiotensin-converting enzyme
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: Ace
• UniProt Synonym Gene Names: Dcp1; ACE
• UniProt Entry Name: ACE_RAT

NCBI Description

catalyzes the conversion of angiotensin I to angiotensin II; plays a role in regulation of blood pressure [RGD, Feb 2006]

Uniprot Description

Function: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm

By similarity.

Catalytic activity: Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.

Cofactor: Binds 2 zinc ions per subunit. Isoform Testis-specific only binds 1 zinc ion per subunit

By similarity.Binds 3 chloride ions per subunit

By similarity.

Subcellular location: Angiotensin-converting enzyme, soluble form: Secreted

By similarity. Cell membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Testis-specific isoform is expressed in spermatocytes, adult testis. Also expressed in brain, kidney, lung, skeletal muscle and heart. Ref.3

Induction: Up-regulated after myocardial infarction. Ref.6

Post-translational modification: Phosphorylated by CK2 on Ser-1306; which allows membrane retention

By similarity.

Sequence similarities: Belongs to the peptidase M2 family.