APRIL Active Protein | ANP32B active protein

APRIL, Recombinant, Mouse (a Proliferation Inducing Ligand, TALL-2, TNF and ApoL-related Leukocyte-expressed Ligand 2, TRDL-1a, TNF-related Death Ligand 1a, TNFSF13)

Cat. Num. AAA650656

• Gene Names: ANP32B; APRIL; SSP29; PHAPI2
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC analyses Endotoxin: 0.1ng/ug (1EU/ug)
• Synonyms: APRIL; Recombinant; Mouse (a Proliferation Inducing Ligand; TALL-2; TNF and ApoL-related Leukocyte-expressed Ligand 2; TRDL-1a; TNF-related Death Ligand 1a; TNFSF13); ANP32B active protein

• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC analyses Endotoxin: 0.1ng/ug (1EU/ug)
• Form/Format: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 5, 100mM arginine. Reconstitute in sterile dH2O to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers with carrier protein. Do not vortex..
• Sequence: MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL

• Biological Activity: Measured by its ability to induce cell proliferation of activated T cells.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with ddH2O, 0.1% BSA or HSA. Aliquot and store at -20 degree C. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ANP32B active protein

APRIL (a proliferation-inducing Ligand), also known as TNFSF13, is a type II membrane protein belonging to the TNF Ligand family. Although low levels of APRIL are found in normal tissues, it is abundantly expressed in tumor cells. Recombinant soluble APRIL stimulates proliferation in Raji B cells, Jurkat T cells and MCF-7 carcinoma cells. Both APRIL and BAFF (BLyS) mediate their distinct biological roles through two receptors, TACI and BCMA. Recombinant Murine APRIL is a soluble 21.9kD protein, consisting of 192 amino acid residues.

Product Categories/Family for ANP32B active protein

Growth Factors; Cytokines; Growth Factors-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 5454088
• NCBI GeneID: 10541
• NCBI Accession #: NP_006392.1
• NCBI GenBank Nucleotide #: NM_006401.2
• UniProt Accession #: Q92688; O00655; P78458; P78459; B2R9C7
• Molecular Weight: 21.9kD
• NCBI Official Full Name: acidic leucine-rich nuclear phosphoprotein 32 family member B
• NCBI Official Synonym Full Names: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• NCBI Official Symbol: ANP32B
• NCBI Official Synonym Symbols: APRIL; SSP29; PHAPI2
• NCBI Protein Information: acidic leucine-rich nuclear phosphoprotein 32 family member B; silver-stainable protein SSP29; acidic protein rich in leucines; putative HLA-DR-associated protein I-2
• UniProt Protein Name: Acidic leucine-rich nuclear phosphoprotein 32 family member B
• Protein Family: Acidic leucine-rich nuclear phosphoprotein 32 family
• UniProt Gene Name: ANP32B
• UniProt Synonym Gene Names: APRIL; PHAPI2; PHAPI2
• UniProt Entry Name: AN32B_HUMAN

Uniprot Description

Function: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity

By similarity. Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Ref.15

Subunit structure: Monomer. Interacts with histones H3 and H4. Ref.15

Subcellular location: Isoform 1: Nucleus. Note: Accumulates in the nuclei at the S phase

By similarity.Isoform 2: Cytoplasm. Note: Lacks a nuclear localization signal.

Tissue specificity: Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta. Ref.3

Domain: Histone binding is mediated by the concave surface of the LRR region.

Post-translational modification: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group

By similarity.

Sequence similarities: Belongs to the ANP32 family.Contains 4 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.