Vascular Endothelial Growth Factor, Endocrine-gland-Derived Active Protein
Vascular Endothelial Growth Factor, Endocrine-gland-Derived, Recombinant, Human (EG-VEGF)
Purity
Highly Purified98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug)
Synonyms
Vascular Endothelial Growth Factor; Endocrine-gland-Derived; Recombinant; Human (EG-VEGF); Endocrine-gland-Derived active protein
Host
Recombinant, Human (E Coli).
Purity/Purification
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug)
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug)
Form/Format
Supplied as a lyophilized powder with no additives. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. to a concentration of >0.1mg/ml
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Ile-Thr-Gly.
Biological Activity
The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-5ng/ml.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of EG-VEGF as a Reference Standard.
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of EG-VEGF as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% BSA or HSA and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Vascular Endothelial Growth Factor, Endocrine-gland-Derived active protein
Human endocrine gland derived vascular endothelial growth factor (EG-VEGF) is selectively expressed in steroidogenic glands and promotes growth of endocrine gland endothelium. The identification of tissue-selective angiogenic factors raises the possibility that other secreted molecules in this class exist. Consistent with such an expression pattern, the human EG-VEGF gene promoter has a potential binding site for steroidogenic factor (SF)-1, a pivotal element for steroidogenic-specific transcription. In the human ovary, the expression of EG-VEGF is temporally and spatially complementary to the expression of VEGF-A, both in the follicular and in the luteal phase, suggesting complementary and coordinated roles of these molecules in ovarian angiogenesis. Also, EG-VEGF expression correlates with vascularity in the polycystic ovary syndrome, a leading cause of infertility.
Recombinant Human EG-VEGF is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.6kD.
Recombinant Human EG-VEGF is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.6kD.
Product Categories/Family for Vascular Endothelial Growth Factor, Endocrine-gland-Derived active protein
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Product Notes
The Vascular Endothelial Growth Factor, Endocrine-gland-Derived (Catalog #AAA650408) is an Active Protein produced from Recombinant, Human (E Coli). and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Il e-Thr-Gly. It is sometimes possible for the material contained within the vial of "Vascular Endothelial Growth Factor, Endocrine-gland-Derived, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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