Herpesvirus Entry Mediator Active Protein | Tnfrsf14 active protein

Recombinant Mouse Herpesvirus Entry Mediator Protein

Cat. Num. AAA2556747

• Gene Names: Tnfrsf14; TR2; Atar; HveA; Hvem; Tnfrs14
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Herpesvirus Entry Mediator; Recombinant Mouse Herpesvirus Entry Mediator Protein; Tnfrsf14; Herpesvirus entry mediator; HVEM; TR2; TNF receptor-like molecule; ATAR; another TRAF-associated receptor; Tumor Necrosis Factor Receptor Superfamily Member 14; Tnfrsf14 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence Length: 275

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Gln 206
• Fusion Tag: C-Fc
• Activity: Measured by its binding ability in a functional ELISA.Immobilized mouse HVEM-Fcat 10 mug/mL (100 mul/well) can bind biotinylated mouse BTLA-Fc,The EC50 of biotinylated mouse BTLA-Fc is 152-228 ng/mL.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-Page

Related Product Information for Tnfrsf14 active protein

Protein Construction: Recombinant Mouse Herpesvirus Entry Mediator is produced by our Mammalian expression system and the target gene encoding Gln39-Val207 is expressed with a Fc tag at the C-terminus.

Background: Mouse Protein Tnfrsf14, is a type I transmembrane protein belonging to the TNF receptor superfamily. It is tumor necrosis factor receptor superfamily member 14 and expressed on the surface of T cells during the resting state. Interaction of HVEM with TNF family member LIGHT co-stimulates T cells and promotes inflammation. HVEM also triggers inhibitory signaling cascade in effector T (Teff) cells and regulatory T cells (Tregs) as a ligand of B and T lymphocyte attenuator. Tnfrsf14 is detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. It has demonstrated that HVEM Ig is able to exert a significant antiviral effect against HSV-1 infection in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 30725810
• NCBI GeneID: 230979
• NCBI Accession #: NP_849262.1
• NCBI GenBank Nucleotide #: NM_178931.2
• UniProt Accession #: Q80WM9
• Molecular Weight: Molecular Mass: 45.6kDa; Actual Protein Molecular Mass: 50 & 60kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 14
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• NCBI Official Symbol: Tnfrsf14
• NCBI Official Synonym Symbols: TR2; Atar; HveA; Hvem; Tnfrs14
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 14
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 14
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf14
• UniProt Synonym Gene Names: Herpesvirus entry mediator ABy similarityManual assertion inferred from sequence similarity toiUniProtKB:Q92956Â (TNR14_HUMAN); HveABy similarityManual assertion inferred from sequence similarity toiUniProtKB:Q92956Â (TNR14_HUMAN); TR2By similarityManual

Uniprot Description

Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation (PubMed:19915044). Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response (PubMed:25711213). In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and proinflammatory cytokines (PubMed:22801499). Upon binding to CD160 on activated CD4+ T cells, downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (). May interact in cis (on the same cell) or in trans (on other cells) with BTLA (PubMed:19915044, PubMed:15568026). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044, PubMed:15568026).