Rabbit anti-Human PRMT5 Polyclonal Antibody | anti-PRMT5 antibody

PRMT5, CT (C487) (Protein Methyltransferase JBP1, Protein Arginine N-methyltransferase 5, Histone-arginine N-methyltransferase PRMT5, Shk1 Kinase-binding Protein 1 Homolog, SKB1 Homolog, SKB1Hs, Jak-binding Protein 1, 72kD ICln-Binding Protein, HRMT1L5, I

Cat. Num. AAA6494427

• Gene Names: PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PRMT5; Polyclonal Antibody; CT (C487) (Protein Methyltransferase JBP1; Protein Arginine N-methyltransferase 5; Histone-arginine N-methyltransferase PRMT5; Shk1 Kinase-binding Protein 1 Homolog; SKB1 Homolog; SKB1Hs; Jak-binding Protein 1; 72kD ICln-Binding Protein; HRMT1L5; I; anti-PRMT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT5.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PRMT5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PRMT5 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of anti-PRMT5 Antibody (C-term C487) in HL60 cell line lysates (35ug/lane).PRMT5(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PRMT5 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003). 2. Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001). 3. Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999). 4. Gilbreth, M., et al., Proc. Natl. Acad. Sci. USA 95(25):14781-14786 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 20070220
• NCBI GeneID: 10419
• NCBI Accession #: NP_006100
• NCBI GenBank Nucleotide #: NM_006109
• UniProt Accession #: O14744; Q6IBR1; Q9UKH1; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7
• Molecular Weight: 66,311 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 5 isoform a
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; SKB1 homolog; histone-arginine N-methyltransferase PRMT5; jak-binding protein 1; shk1 kinase-binding protein 1 homolog
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN

Uniprot Description

PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

Protein type: EC 2.1.1.125; Cell cycle regulation; Methyltransferase, protein arginine; RNA processing; Methyltransferase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity

Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; peptidyl-arginine N-methylation; endothelial cell activation