Rabbit Synaptophysin/SYP Polyclonal Antibody | anti-SYP antibody

Anti-Synaptophysin/SYP Antibody, Rabbit Polyclonal

Cat. Num. AAA8100133

• Gene Names: SYP; MRXSYP
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Synaptophysin/SYP; Polyclonal Antibody; Anti-Synaptophysin/SYP Antibody; Rabbit Polyclonal; Synaptophysin Antibody; Rabbit PAb; Antigen Affinity Purified; synaptophysin; Anti-MRX96 Antibody; Anti-MRXSYP Antibody; anti-SYP antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Synaptophysin/SYP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SYP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human Synaptophysin
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the C-terminus of the human Synaptophysin, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Synaptophysin rabbit polyclonal antibody at 1:500 dilutionLane A: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:43 kDa)

Related Product Information for anti-SYP antibody

Synaptophysin (SYP) is an integral membrane protein of synaptic vesicles and is ubiquitously expressed in neurons throughout the brain, such as small presynaptic vesicles of the nerve cells and in microvesicles of the neuroendocrine cells. SYP is a major protein of neurotransmitter-containing vesicles spanning the membrane four times and contributing to various aspects of the synaptic vesicle cycle. SYP is involved in the release of neurotransmitters, synaptic plasticity, and the formation and recycling of synaptic vesicles. SYP may serve as a specific marker of synaptic proteins. As Syp expression is correlated with synaptogenesis during the development of the central nervous system, the expression of Syp is considered to be a critical aspect of neuronal maturation and circuit formation.

References

Li Q, et al. (2014) Inhibition of synaptophysin ubiquitination may improve the intelligent drop due to high glucose and hypoxia. Int J Clin Exp Med 7 (12): 5021-5030.Kolos YA, et al. (2015) [a synaptic marker synaptophysin]. Morfologiia 147 (1): 78-82.Felkl M, et al. (2008) Interaction assays in yeast and cultured cells confirm known and identify novel partners of the synaptic vesicle protein synaptophysin. Neuroscience 156 (2): 344-352.Aizawa S, et al. (2020) Possible involvement of DNA methylation in hippocampal synaptophysin gene expression during postnatal development of mice. Neurochem Int 132 104587.

NCBI and Uniprot Product Information

• NCBI GI #: 27764867
• NCBI GeneID: 6855
• NCBI Accession #: NP_003170.1
• NCBI GenBank Nucleotide #: NM_003179.2
• UniProt Accession #: P08247; Q6P2F7; B2R7L6
• Molecular Weight: 33,845 Da
• NCBI Official Full Name: synaptophysin
• NCBI Official Synonym Full Names: synaptophysin
• NCBI Official Symbol: SYP
• NCBI Official Synonym Symbols: MRXSYP
• NCBI Protein Information: synaptophysin; major synaptic vesicle protein P38
• UniProt Protein Name: Synaptophysin
• Protein Family: Synaptophysin
• UniProt Gene Name: SYP
• UniProt Entry Name: SYPH_HUMAN

NCBI Description

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Uniprot Description

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: Xp11.23-p11.22

Cellular Component: presynaptic membrane; synaptic vesicle; neuron projection; protein complex; synaptic vesicle membrane; presynaptic active zone; excitatory synapse; cell junction; integral to synaptic vesicle membrane

Molecular Function: identical protein binding; protein self-association; transporter activity; protein complex binding; cholesterol binding; syntaxin-1 binding; SH2 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity

Disease: Mental Retardation, X-linked 96