Rabbit anti-Human PECI Polyclonal Antibody | anti-PECI antibody

PECI, CT (Peroxisomal 3,2-trans-enoyl-CoA Isomerase, ACBD2, Delta(3),delta(2)-enoyl-CoA Isomerase, D3,D2-enoyl-CoA Isomerase, Diazepam-binding Inhibitor-related Protein 1, DBI-related Protein 1, dJ1013A10.3, Dodecenoyl-CoA Isomerase, DRS1, DRS-1, Hepatoce

Cat. Num. AAA6492570

• Gene Names: ECI2; DRS1; PECI; ACBD2; DRS-1; HCA88; dJ1013A10.3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PECI; Polyclonal Antibody; CT (Peroxisomal 3; 2-trans-enoyl-CoA Isomerase; ACBD2; Delta(3); delta(2)-enoyl-CoA Isomerase; D3; D2-enoyl-CoA Isomerase; Diazepam-binding Inhibitor-related Protein 1; DBI-related Protein 1; dJ1013A10.3; Dodecenoyl-CoA Isomerase; DRS1; DRS-1; Hepatoce; Peroxisomal D3; EC=5.3.3.8; anti-PECI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PECI.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PECI antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PECI (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PECI Antibody (C-term) in HepG2 cell line lysates (35ug/lane).PECI (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PECI Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of PECI (arrow) using rabbit polyclonal PECI Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PECI gene.)

Product Categories/Family for anti-PECI antibody

Antibodies; Enzymes; Isomerase

References

1. Scanlan, M.J., et.al., Int. J. Cancer 83(4), 456-464 (1999). 2. Suk, K., et.al., Biochim. Biophys. Acta 1454(1), 126-131 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 45643119
• NCBI GeneID: 10455
• NCBI Accession #: NP_006108.2
• NCBI GenBank Nucleotide #: NM_006117.2
• UniProt Accession #: O75521; Q5JYK5; Q5JYK7; Q7L124; Q8N0X0; Q9BUE9; Q9H0T9; Q9NQH1; Q9NYH7; Q9UN55
• Molecular Weight: 39,609 Da
• NCBI Official Full Name: enoyl-CoA delta isomerase 2, mitochondrial isoform 1
• NCBI Official Synonym Full Names: enoyl-CoA delta isomerase 2
• NCBI Official Symbol: ECI2
• NCBI Official Synonym Symbols: DRS1; PECI; ACBD2; DRS-1; HCA88; dJ1013A10.3
• NCBI Protein Information: enoyl-CoA delta isomerase 2, mitochondrial; DBI-related protein 1; dodecenoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; renal carcinoma antigen NY-REN-1; delta(3),delta(2)-enoyl-CoA isomerase; peroxisomal D3,D2-enoyl-CoA isomerase; peroxisomal 3,2-trans-e
• UniProt Protein Name: Enoyl-CoA delta isomerase 2, mitochondrial
• UniProt Gene Name: ECI2
• UniProt Synonym Gene Names: DRS1; HCA88; PECI; D3,D2-enoyl-CoA isomerase; DBI-related protein 1; pECI
• UniProt Entry Name: ECI2_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]

Uniprot Description

PECI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - fatty acid; Isomerase; EC 5.3.3.8

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: nucleoplasm; peroxisomal matrix; membrane; intracellular membrane-bound organelle; mitochondrion

Molecular Function: acyl-CoA binding; dodecenoyl-CoA delta-isomerase activity; receptor binding

Biological Process: metabolic process; fatty acid catabolic process