Mouse anti-Human PEPD Monoclonal Antibody | anti-PEPD antibody

PEPD (Xaa-Pro Dipeptidase, X-Pro Dipeptidase, Proline Dipeptidase, Prolidase, Imidodipeptidase, Peptidase D, PRD, MGC10905) (AP)

Cat. Num. AAA6132877

• Gene Names: PEPD; PROLIDASE
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PEPD; Monoclonal Antibody; PEPD (Xaa-Pro Dipeptidase; X-Pro Dipeptidase; Proline Dipeptidase; Prolidase; Imidodipeptidase; Peptidase D; PRD; MGC10905) (AP); anti-PEPD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1D5-H3
• Specificity: Recognizes human PEPD.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1917

• Applicable Applications for anti-PEPD antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-494 from human PEPD (AAH15027) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVAEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQEPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (79.97kD).)

Western Blot (WB)

(PEPD monoclonal antibody Western Blot analysis of PEPD expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody. Lane 1: PEPD transfected lysate (Predicted MW: 54.6kD). Lane 2: Non-transfected lysate)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10ug/ml])

Immunoprecipitation (IP)

(Immunoprecipitation of PEPD transfected lysate using PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged PEPD is ~0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-PEPD antibody

Antibodies; Enzymes; Peptidase

NCBI and Uniprot Product Information

• NCBI GI #: 15929142
• NCBI GeneID: 5184
• NCBI Official Full Name: Homo sapiens peptidase D, mRNA
• NCBI Official Synonym Full Names: peptidase D
• NCBI Official Symbol: PEPD
• NCBI Official Synonym Symbols: PROLIDASE
• NCBI Protein Information: xaa-Pro dipeptidase
• Protein Family: Dipeptidase

NCBI Description

This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]