SDS-Page
Endoglin/CD105 Active Protein | ENG active protein
Endoglin/CD105 Protein, Mouse, Recombinant (His Tag)
Purity
>97% as determined by SDS-PAGE
Synonyms
Endoglin/CD105; Endoglin/CD105 Protein; Mouse; Recombinant (His Tag); Mouse Endoglin/CD105/ENG Protein (His Tag); AI528660 Protein; AI662476 Protein; CD105 Protein; Endo Protein; S-endoglin Protein; endoglin; ENG active protein
Host
HEK293 Cells
Purity/Purification
>97% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Gly580
Species
Mouse
Activity
1. Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. The ED50 for this effect is typically 25-100ng/mL in the presence of 2ng/mL of recombiant human BMP9.
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Arg 27
Tag
C-His
Protein Construction
A DNA sequence encoding the mouse CD105 (NP_031958.2) extracellular domain (Met 1-Gly 580) was expressed, with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Testing Data
(Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells.The ED50 for this effect is typically 25-100 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.)
Testing Data
(Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells.The ED50 for this effect is typically 25-100 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.)
Related Product Information for ENG active protein
Background: Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-beta superfamily ligands, endoglin binds TGF-beta1 and TGF-beta3 with high affinity not by itself but by associating with TGF-beta type II receptor (TbetaRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Product Categories/Family for ENG active protein
References
Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.
NCBI and Uniprot Product Information
Molecular Weight
The secreted recombinant mouse CD105 consists of 565 amino acids and has a calculated molecular mass of 61.2kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 65-70kDa in SDS-PAGE under reducing cond
Protein Family
Similar Products
Product Notes
The ENG (Catalog #AAA8122724) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Gly58 0. It is sometimes possible for the material contained within the vial of "Endoglin/CD105, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
