Rabbit JAK1 Polyclonal Antibody | anti-JAK1 antibody

JAK1 (Janus Kinase 1, JAK-1, JAK1A, JAK1B, JTK3, Tyrosine-protein Kinase JAK1) (MaxLight 405)

Cat. Num. AAA6483705

• Gene Names: JAK1; JTK3; JAK1A; JAK1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: JAK1; Polyclonal Antibody; JAK1 (Janus Kinase 1; JAK-1; JAK1A; JAK1B; JTK3; Tyrosine-protein Kinase JAK1) (MaxLight 405); anti-JAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human JAK1 at ~130kD. Does not react with JAK2. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-JAK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000 detects JAK1 on 10ug of PC12 lysates.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acids 1123-1141, CQPSNRTSFQNLIEGFEALLK of human JAK1. Cellular Localization: Intracytoplasmic membrane; Peripheral membrane protein.
• Conjugate: MaxLight405
• Positive Control: PC12 Cell Lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot: PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620116 Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein JAK1 (~130kD).)

Western Blot (WB)

(Western Blot: PC12 lysate (Lane 1) and 3T3/L1 lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with A0900-05C. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein JAK1 (~130kD).)

Product Categories/Family for anti-JAK1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 102469034
• NCBI GeneID: 3716
• NCBI Accession #: NP_002218.2
• NCBI GenBank Nucleotide #: NM_002227.2
• UniProt Accession #: P23458; Q59GQ2; Q9UD26
• Molecular Weight: 133,277 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK1
• NCBI Official Synonym Full Names: Janus kinase 1
• NCBI Official Symbol: JAK1
• NCBI Official Synonym Symbols: JTK3; JAK1A; JAK1B
• NCBI Protein Information: tyrosine-protein kinase JAK1
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK1
• UniProt Synonym Gene Names: JAK1A; JAK1B; JAK-1
• UniProt Entry Name: JAK1_HUMAN

NCBI Description

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. [provided by RefSeq, Jul 2008]

Uniprot Description

JAK1: a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways. Couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. A selective Jak1 inhibitor induces apoptosis in NRP-154 prostate cancer cell line. A single mutation seen in tyrosine kinome screen of colon cancers. Activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. Inhibitor: Piceatannol.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; JakA family

Chromosomal Location of Human Ortholog: 1p32.3-p31.3

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; focal adhesion; cytoplasm; endomembrane system; nucleus; cytosol

Molecular Function: protein binding; growth hormone receptor binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; CCR5 chemokine receptor binding

Biological Process: response to antibiotic; cell migration; cytokine and chemokine mediated signaling pathway; innate immune response; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation