Rabbit JAK1 Polyclonal Antibody | anti-JAK1 antibody

JAK1 (Janus Kinase 1, JAK-1, JAK1A, JAK1B, JTK3, Tyrosine-protein Kinase JAK1) (AP)

Cat. Num. AAA6483700

• Gene Names: JAK1; JTK3; JAK1A; JAK1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: JAK1; Polyclonal Antibody; JAK1 (Janus Kinase 1; JAK-1; JAK1A; JAK1B; JTK3; Tyrosine-protein Kinase JAK1) (AP); anti-JAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human JAK1 at ~130kD. Does not react with JAK2. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-JAK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000 detects JAK1 on 10ug of PC12 lysates.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acids 1123-1141, CQPSNRTSFQNLIEGFEALLK of human JAK1. Cellular Localization: Intracytoplasmic membrane; Peripheral membrane protein.
• Conjugate: AP
• Positive Control: PC12 Cell Lysate
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot: PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620116 Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein JAK1 (~130kD).)

Western Blot (WB)

(Western Blot: PC12 lysate (Lane 1) and 3T3/L1 lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with A0900-05C. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein JAK1 (~130kD).)

Product Categories/Family for anti-JAK1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 102469034
• NCBI GeneID: 3716
• NCBI Accession #: NP_002218.2
• NCBI GenBank Nucleotide #: NM_002227.2
• UniProt Accession #: P23458; Q59GQ2; Q9UD26
• Molecular Weight: 133,277 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK1
• NCBI Official Synonym Full Names: Janus kinase 1
• NCBI Official Symbol: JAK1
• NCBI Official Synonym Symbols: JTK3; JAK1A; JAK1B
• NCBI Protein Information: tyrosine-protein kinase JAK1
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK1
• UniProt Synonym Gene Names: JAK1A; JAK1B; JAK-1
• UniProt Entry Name: JAK1_HUMAN

NCBI Description

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. [provided by RefSeq, Jul 2008]

Uniprot Description

JAK1: a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways. Couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. A selective Jak1 inhibitor induces apoptosis in NRP-154 prostate cancer cell line. A single mutation seen in tyrosine kinome screen of colon cancers. Activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. Inhibitor: Piceatannol.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; JakA family

Chromosomal Location of Human Ortholog: 1p32.3-p31.3

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; focal adhesion; cytoplasm; endomembrane system; nucleus; cytosol

Molecular Function: protein binding; growth hormone receptor binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; CCR5 chemokine receptor binding

Biological Process: response to antibiotic; cell migration; cytokine and chemokine mediated signaling pathway; innate immune response; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation