Human Tyrosine-protein kinase JAK1 ELISA Kit | JAK1 elisa kit

Human Tyrosine-protein kinase JAK1 ELISA Kit

Cat. Num. AAA761284

• Gene Names: JAK1; JTK3; JAK1A; JAK1B
• Reactivity: Human
• Synonyms: Tyrosine-protein kinase JAK1; Human Tyrosine-protein kinase JAK1 ELISA Kit; JAK1/Janus Kinase 1/EC 2.7.10/JAK-1/JAK1Atyrosine-protein kinase JAK1/JAK1B/Janus kinase 1EC 2.7.10.2/JTK3; JAK1 elisa kit

• Reactivity: Human
• Sequence Length: 1154

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for JAK1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human JAK1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human JAK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human JAK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human JAK1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 102469034
• NCBI GeneID: 3716
• NCBI Accession #: NP_002218.2
• NCBI GenBank Nucleotide #: NM_002227.2
• Molecular Weight: 133,277 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK1
• NCBI Official Synonym Full Names: Janus kinase 1
• NCBI Official Symbol: JAK1
• NCBI Official Synonym Symbols: JTK3; JAK1A; JAK1B
• NCBI Protein Information: tyrosine-protein kinase JAK1
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK1
• UniProt Synonym Gene Names: JAK1A; JAK1B; JAK-1
• UniProt Entry Name: JAK1_HUMAN

NCBI Description

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. [provided by RefSeq, Jul 2008]

Uniprot Description

JAK1: a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways. Couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. A selective Jak1 inhibitor induces apoptosis in NRP-154 prostate cancer cell line. A single mutation seen in tyrosine kinome screen of colon cancers. Activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. Inhibitor: Piceatannol.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; TK group; JakA family

Chromosomal Location of Human Ortholog: 1p32.3-p31.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; endomembrane system; extrinsic to internal side of plasma membrane; focal adhesion; nucleus

Molecular Function: ATP binding; CCR5 chemokine receptor binding; growth hormone receptor binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor binding; ubiquitin protein ligase binding

Biological Process: activation of MAPKK activity; axon guidance; cell differentiation; cell migration; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; protein amino acid phosphorylation; Ras protein signal transduction; regulation of cell proliferation; response to antibiotic; small GTPase mediated signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway