Rabbit anti-Human, Mouse HDAC11 Polyclonal Antibody | anti-HDAC11 antibody

HDAC11, CT (Histone Deacetylase 11, HD11) (APC)

Cat. Num. AAA6479620

• Gene Names: HDAC11; HD11
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HDAC11; Polyclonal Antibody; CT (Histone Deacetylase 11; HD11) (APC); anti-HDAC11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HDAC11. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HDAC11 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human HDAC11 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-HDAC11 Pab is used in Western blot to detect HDAC11 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Purified rabbit anti-HDAC11 antibody (C-term) was used in immunohistochemistry on Breast Carcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testicle tumor tissue reacted with HDAC11 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-HDAC11 antibody

Antibodies; Enzymes; Deacetylase

References

1. Voelter-Mahlknecht S, et al., Int J Mol Med. 2005 Oct;16(4):589-98. 2. Bradbury CA, et al., Leukemia. 2005 Oct;19(10):1751-9. 3. Gregoretti IV, et al., J Mol Biol. 2004 Apr 16;338(1):17-31. 4. Gao, L., et al., J. Biol. Chem. 277(28):25748-25755 (2002). 5. Kara S. Keedy, et al. J. Virol. 2009; 83:4749-4756. (E-pub) Keedy KS, et al. J Virol. 2009 May;83(10):4749-56. (Applications: WB)(E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 217272877
• NCBI GeneID: 79885
• NCBI Accession #: NP_079103.2
• NCBI GenBank Nucleotide #: NM_024827.3
• UniProt Accession #: Q96DB2; Q9H6I7; Q9H6X3; Q9NTC9; B4DDK1
• Molecular Weight: 33,124 Da
• NCBI Official Full Name: histone deacetylase 11 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 11
• NCBI Official Symbol: HDAC11
• NCBI Official Synonym Symbols: HD11
• NCBI Protein Information: histone deacetylase 11
• UniProt Protein Name: Histone deacetylase 11
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC11
• UniProt Synonym Gene Names: HD11

NCBI Description

This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2009]

Uniprot Description

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.