Rabbit anti-Human Exportin T Polyclonal Antibody | anti-XPOT antibody

Exportin T, CT (tRNA Exportin, XPOT) (HRP)

Cat. Num. AAA6475455

• Gene Names: XPOT; XPO3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Exportin T; Polyclonal Antibody; CT (tRNA Exportin; XPOT) (HRP); anti-XPOT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XPOT.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-XPOT antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human XPOT (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of XPOT antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). XPOT (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with XPOT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(XPOT Antibody (C-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-XPOT antibody

Antibodies; Proteins

References

1. Li, S., RNA Biol 3(4), 145-149 (2006). 2. Suzuki, T., Biomed. Res. 27(2), 89-92 (2006). 3. Kuersten, S., Mol. Cell. Biol. 22(16), 5708-5720 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 8051636
• NCBI GeneID: 11260
• NCBI Accession #: NP_009166
• NCBI GenBank Nucleotide #: NM_007235
• UniProt Accession #: O43592
• Molecular Weight: 110kDa
• NCBI Official Full Name: exportin-T
• NCBI Official Synonym Full Names: exportin for tRNA
• NCBI Official Symbol: XPOT
• NCBI Official Synonym Symbols: XPO3
• NCBI Protein Information: exportin-T
• UniProt Protein Name: Exportin-T
• UniProt Gene Name: XPOT
• UniProt Entry Name: XPOT_HUMAN

NCBI Description

This gene encodes a protein belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN. [provided by RefSeq, Jul 2008]

Uniprot Description

exportin T: Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP- bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor. XPOT then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the exportin family.

Protein type: Nuclear import; Karyopherin; Nuclear export

Chromosomal Location of Human Ortholog: 12q14.2

Cellular Component: nucleoplasm; cytoplasm; nuclear pore

Molecular Function: Ran GTPase binding; tRNA binding

Biological Process: intracellular protein transport; tRNA export from nucleus