Immunohistochemistry (IHC)
(MBS9608375 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse TNFSF4 Polyclonal Antibody | anti-TNFSF4 antibody
TNFSF4 Antibody
Gene Names
TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TNFSF4; Polyclonal Antibody; TNFSF4 Antibody; CD 134L; CD 252; CD134; CD134 ligand; CD134L; CD252; CD252 antigen; glycoprotein 34 kd; Glycoprotein Gp34; GP34; OX-40L; OX40 antigen ligand; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; TNFL4_HUMAN; Tnfsf4; Tumor necrosis factor (ligand) superfamily member 4; Tumor necrosis factor ligand superfamily member 4; TXGP1; anti-TNFSF4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TNFSF4 antibody detects endogenous levels of total TNFSF4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
133
Applicable Applications for anti-TNFSF4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human TNFSF4
Subcellular Location
Membrane.
Tissue Specificity
By HTLV-1 transactivator p40-Tax.
Predicted Cross Reactivity
Rabbit
Similarity
Rabbit (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9608375 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9608375 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of TNFSF4 using COS7 whole lysates.)
Western Blot (WB)
(Western blot analysis of TNFSF4 using COS7 whole lysates.)
Related Product Information for anti-TNFSF4 antibody
Function: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Subunit Structure: Homotrimer.
Similarity: Belongs to the tumor necrosis factor family.
Subunit Structure: Homotrimer.
Similarity: Belongs to the tumor necrosis factor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 21 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 4 isoform 2
NCBI Official Synonym Full Names
TNF superfamily member 4
NCBI Official Symbol
TNFSF4
NCBI Official Synonym Symbols
GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
NCBI Protein Information
tumor necrosis factor ligand superfamily member 4
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 4
Protein Family
UniProt Gene Name
TNFSF4
UniProt Synonym Gene Names
TXGP1; OX40L
NCBI Description
This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Research Articles on TNFSF4
Similar Products
Product Notes
The TNFSF4 tnfsf4 (Catalog #AAA9608375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNFSF4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNFSF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the TNFSF4 tnfsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFSF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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