Guinea Pig Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1) ELISA Kit | KCNQ1 elisa kit

Guinea pig Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1) ELISA Kit

Cat. Num. AAA7246734

• Gene Names: KCNQ1; LQT; RWS; WRS; LQT1; SQT2; ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1
• Reactivity: Guinea Pig
• Synonyms: Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1); Guinea pig Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1) ELISA Kit; KCNQ1 elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This KCNQ1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig KCNQ1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KCNQ1 elisa kit

Principle of the assay: KCNQ1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KCNQ1 antibody and an KCNQ1-HRP conjugate. The assay sample and buffer are incubated together with KCNQ1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KCNQ1 concentration since KCNQ1 from samples and KCNQ1-HRP conjugate compete for the anti-KCNQ1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KCNQ1 from the sample, fewer sites are left to bind KCNQ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KCNQ1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6166005
• NCBI GeneID: 3784
• Molecular Weight: 61,474 Da
• NCBI Official Full Name: Potassium voltage-gated channel subfamily KQT member 1
• NCBI Official Synonym Full Names: potassium voltage-gated channel, KQT-like subfamily, member 1
• NCBI Official Symbol: KCNQ1
• NCBI Official Synonym Symbols: LQT; RWS; WRS; LQT1; SQT2; ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1
• NCBI Protein Information: potassium voltage-gated channel subfamily KQT member 1; slow delayed rectifier channel subunit; voltage-gated potassium channel subunit Kv7.1; kidney and cardiac voltage dependend K+ channel; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1
• UniProt Protein Name: Potassium voltage-gated channel subfamily KQT member 1
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNQ1
• UniProt Synonym Gene Names: KCNA8; KCNA9; KVLQT1
• UniProt Entry Name: KCNQ1_HUMAN

NCBI Description

This gene encodes a voltage-gated potassium channel required for repolarization phase of the cardiac action potential. This protein can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome 1 (also known as Romano-Ward syndrome), Jervell and Lange-Nielsen syndrome, and familial atrial fibrillation. This gene exhibits tissue-specific imprinting, with preferential expression from the maternal allele in some tissues, and biallelic expression in others. This gene is located in a region of chromosome 11 amongst other imprinted genes that are associated with Beckwith-Wiedemann syndrome (BWS), and itself has been shown to be disrupted by chromosomal rearrangements in patients with BWS. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Kv7.1: a voltage-gated potassium channel protein required for the repolarization phase of the cardiac action potential. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current. May associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and pathologically stimulated in cholera and other forms of secretory diarrhea. Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: voltage-gated potassium channel complex; cytoplasmic vesicle membrane; basolateral plasma membrane; lysosome; late endosome; early endosome; plasma membrane

Molecular Function: calmodulin binding; voltage-gated potassium channel activity; protein binding; delayed rectifier potassium channel activity; protein phosphatase 1 binding

Biological Process: synaptic transmission; genetic imprinting; sensory perception of sound; positive regulation of heart rate; gene silencing; regulation of heart contraction; cardiac muscle contraction

Disease: Jervell And Lange-nielsen Syndrome 1; Beckwith-wiedemann Syndrome; Atrial Fibrillation, Familial, 3; Short Qt Syndrome 2; Long Qt Syndrome 1