Immunoprecipitation (IP)
(Immunoprecipitation of MGEA5 transfected lysate using MGEA5 rabbit polyclonal antibody and Protein A Magnetic Bead and immunoblotted with MGEA5 mouse polyclonal antibody.)
Rabbit anti-Human MGEA5 Polyclonal Antibody | anti-MGEA5 antibody
MGEA5 (Bifunctional Protein NCOAT, Meningioma-expressed Antigen 5, Nuclear Cytoplasmic O-GlcNAcase and Acetyltransferase, HEXC, KIAA0679, MEA5, FLJ11229, FLJ23355)
Serum
Serum
Immunoprecipitation (IP)
(Immunoprecipitation of MGEA5 transfected lysate using MGEA5 rabbit polyclonal antibody and Protein A Magnetic Bead and immunoblotted with MGEA5 mouse polyclonal antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of MGEA5 transfected lysate using MGEA5 rabbit polyclonal antibody and Protein A Magnetic Bead and immunoblotted with MGEA5 mouse polyclonal antibody.)
NCBI and Uniprot Product Information
NCBI Description
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. Ref.3 Ref.9
Catalytic activity: [Protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine. Ref.3 Ref.9[Protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine.Acetyl-CoA + [histone] = CoA + acetyl-[histone]. Ref.3 Ref.9
Enzyme regulation: Inhibited by N-acetylglucosamine and not N-acetylgalactosamine. Ref.3
Subunit structure: Monomer. Binds both acetylated and unacetylated histone H4 tail but acetylation on 'Lys-8' of histone H4 abolishes binding
By similarity. Ref.9
Subcellular location: Isoform 3: Nucleus Ref.2 Ref.3 Ref.9. Isoform 1: Cytoplasm Ref.2 Ref.3 Ref.9.
Tissue specificity: Ubiquitous. Shows highest expression in the brain, placenta and pancreas. Ref.2 Ref.3
Post-translational modification: Proteolytically cleaved by caspase-3.
Biophysicochemical propertiesKinetic parameters:KM=1.1 mM for pNP-GLcNAc Ref.3 Ref.9Vmax=652 µmol/min/mg enzyme with pNP-GLcNAc as substratepH dependence:Optimum pH is 5.7-7.
Sequence caution: The sequence AAH47877.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence BAA31654.2 differs from that shown. Reason: Erroneous initiation.
Research Articles on MGEA5
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Product Notes
The MGEA5 mgea5 (Catalog #AAA647206) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGEA5 (Bifunctional Protein NCOAT, Meningioma-expressed Antigen 5, Nuclear Cytoplasmic O-GlcNAcase and Acetyltransferase, HEXC, KIAA0679, MEA5, FLJ11229, FLJ23355) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGEA5 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Suitable for use in Immunoprecipitation. Researchers should empirically determine the suitability of the MGEA5 mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV AGAAGGARRF LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD YKHRMFWREM YSVEEAEQLM TLISAAREYE IEFIYAISPG LDITFSNPKE VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM CAADKEVFSS FAHAQVSITN EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK LLPGIEVLWT GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED STVSIQIKLE NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR QVAHSGAKAS VVDGTPLVAA PSLNATTVVT TVYQEPIMSQ GAALSGEPTT LTKEEEKKQP DEEPMDMVVE KQEETDHKND NQILSEIVEA KMAEELKPMD TDKESIAESK SPEMSMQEDC ISDIAPMQTD EQTNKEQFVP GPNEKPLYTA EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD SEKIEEWRSR AAKFEEMCGL VMGMFTRLSN CANRTILYDM YSYVWDIKSI MSMVKSFVQW LGCRSHSSAQ FLIGDQEPWA FRGGLAGEFQ RLLPIDGAND LFFQPPPLTP TSKVYTIRPY FPKDEASVYK ICREMYDDGV GLPFQSQPDL IGDKLVGGLL SLSLDYCFVL EDEDGICGYA LGTVDVTPFI KKCKISWIPF MQEKYTKPNG DKELSEAEKI MLSFHEEQEV LPETFLANFP SLIKMDIHKK VTDPSVAKSM MACLLSSLKA NGSRGAFCEV RPDDKRILEF YSKLGCFEIA KMEGFPKDVV ILGRSL. It is sometimes possible for the material contained within the vial of "MGEA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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