Western Blot (WB)
(Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human NEK7 Polyclonal Antibody
NEK7, NT (Never in Mitosis A-related Kinase 7, NimA-related Protein Kinase 7, Serine/threonine Protein Kinase Nek7)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Western Blot (WB)
(Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(NEK7 Antibody (M8) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NEK7 antibody detected the NEK7 protein (arrow).)
Western Blot (WB)
(NEK7 Antibody (M8) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NEK7 antibody detected the NEK7 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]
Uniprot Description
NEK7: a protein kinase of the NEK family. Phosphorylates and activates p70S6 kinase.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; NEK family
Chromosomal Location of Human Ortholog: 1q31.3
Cellular Component: nucleoplasm; spindle pole; microtubule; cytoplasm; microtubule organizing center
Molecular Function: protein serine/threonine kinase activity; metal ion binding; ATP binding
Biological Process: regulation of mitotic cell cycle; regulation of signal transduction; cytokinesis; spindle assembly; protein amino acid phosphorylation
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Product Notes
The NEK7 (Catalog #AAA625124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK7, NT (Never in Mitosis A-related Kinase 7, NimA-related Protein Kinase 7, Serine/threonine Protein Kinase Nek7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the NEK7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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