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Western Blot (WB)
(Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.)
Rabbit anti-Human, Mouse MGEA5 Monoclonal Antibody | anti-MGEA5 antibody
MGEA5 Antibody
Gene Names
MGEA5; OGA; MEA5; NCOAT
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
MGEA5; Monoclonal Antibody; MGEA5 Antibody; Beta-hexosaminidase antibody; Beta-N-acetylglucosaminidase antibody; Beta-N-acetylhexosaminidase antibody; Bifunctional protein NCOAT antibody; HEXC antibody; Hyaluronidase in meningioma antibody; KIAA0679 antibody; MEA5 antibody; Meningioma expressed antigen 5 antibody; Meningioma-expressed antigen 5 antibody; MGEA 5 antibody; Mgea5 antibody; N-acetyl-beta-D-glucosaminidase antibody; N-acetyl-beta-glucosaminidase antibody; NCOAT antibody; Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody; OGA antibody; OGA_HUMAN antibody; Protein O-GlcNAcase antibody; anti-MGEA5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
JG40-05
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-MGEA5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:10-1:50
IHC: 1:50-1:200
IP: 1:10-1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein of N-terminal human MGEA5.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.)
Western Blot (WB)
(Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-MGEA5 antibody
Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Product Categories/Family for anti-MGEA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,932 Da
NCBI Official Full Name
protein O-GlcNAcase isoform b
NCBI Official Synonym Full Names
meningioma expressed antigen 5 (hyaluronidase)
NCBI Official Symbol
MGEA5
NCBI Official Synonym Symbols
OGA; MEA5; NCOAT
NCBI Protein Information
protein O-GlcNAcase
UniProt Protein Name
Protein O-GlcNAcase
Protein Family
UniProt Gene Name
MGEA5
UniProt Synonym Gene Names
HEXC; KIAA0679; MEA5; NCOAT
NCBI Description
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
Uniprot Description
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Research Articles on MGEA5
Similar Products
Product Notes
The MGEA5 mgea5 (Catalog #AAA9436701) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGEA5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MGEA5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:10-1:50. Researchers should empirically determine the suitability of the MGEA5 mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGEA5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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