Rabbit anti-Rat FAK Polyclonal Antibody
FAK, Phosphorylated (Tyr397) (Focal Adhesion Kinase 1, FADK 1, pp125FAK, Protein-tyrosine Kinase 2, PTK2, FAK1)
Reactivity
Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
FAK; Polyclonal Antibody; Phosphorylated (Tyr397) (Focal Adhesion Kinase 1; FADK 1; pp125FAK; Protein-tyrosine Kinase 2; PTK2; FAK1); Anti -FAK; anti-FAK antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
Recognizes rat FAK phosphorylated at Tyr397. Species sequence homology: human, xenopus, chicken, mouse (100%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-FAK antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot and Cell Function Assay.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic linear peptide corresponding to human FAK phosphorylated at Tyr397 (KLH).
Positive Control
Untransfected and Src-transfected RAT2 cell lysates
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-FAK antibody
Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.
Product Categories/Family for anti-FAK antibody
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Product Notes
The FAK (Catalog #AAA647130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAK, Phosphorylated (Tyr397) (Focal Adhesion Kinase 1, FADK 1, pp125FAK, Protein-tyrosine Kinase 2, PTK2, FAK1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot and Cell Function Assay. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the FAK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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