Rabbit anti-Human MAP2K5 Polyclonal Antibody | anti-MAP2K5 antibody

MAP2K5 Antibody

Cat. Num. AAA7050140

• Gene Names: MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
• Reactivity: Human
• Applications: ELISA, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: MAP2K5; Polyclonal Antibody; MAP2K5 Antibody; Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5; MEK5; MKK5; PRKMK5; anti-MAP2K5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 412

• Applicable Applications for anti-MAP2K5 antibody: ELISA (EIA), Immunofluorescence (IF)
• Application Notes: IF: 1: 50-1: 200
• Immunogen: Recombinant Human Dual specificity mitogen-activated protein kinase kinase 5 protein (1-190AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10795 / sc-1287 / sc-9320
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysate,HEK293 whole cell lysate,Rat liver tissue,Mouse liver tissueAll lanes: THBD antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 51 KDaObserved band size: 51 KDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7050140 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-MAP2K5 antibody

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 332164798
• NCBI GeneID: 5607
• NCBI Accession #: NP_001193733.1
• NCBI GenBank Nucleotide #: NM_001206804.1
• UniProt Accession #: Q13163; Q92961; Q92962; B4DE43
• Molecular Weight: 46,131 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform C
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 5
• NCBI Official Symbol: MAP2K5
• NCBI Official Synonym Symbols: MEK5; MAPKK5; PRKMK5; HsT17454
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5
• UniProt Entry Name: MP2K5_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]

Uniprot Description

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: cytoplasm

Molecular Function: protein binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of heterotypic cell-cell adhesion; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; signal transduction