FAK recombinant protein

FAK, Active

Cat. Num. AAA517486

• Gene Names: PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• Applications: Western Blot
• Synonyms: FAK; Active; PTK2; FADK; FAK1; FRNK; pp125FAK; FAK recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 0.01% Triton X-100, 25% glycerol.

• Applicable Applications for FAK recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for FAK recombinant protein

Recombinant full-length human FAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The FAK gene accession number is BC035404.
FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation (1). Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness (2). Elevated FAK expression in anaplastic astrocytoma and glioblastoma tumor biopsy samples has been demonstrated.

Product Categories/Family for FAK recombinant protein

Protein Kinases; AKT/PKB Pathway; Angiogenesis; Cancer; Cytoplasmic Tyrosine Kinases; ERK/MAPK Pathway; Invasion/Metastasis

References

1. Schaller, M D.: Biochemical signals and biological responses elicited by the focal adhesion kinase. Biochim Biophys Acta. 2001 Jul 25;1540(1):1-21.
2. Gabarra-Niecko, V. et al: FAK regulates biological processes important for the pathogenesis of cancer. Cancer Metastasis Rev. 2003 Dec;22(4):359-74.

NCBI and Uniprot Product Information

• NCBI GI #: 34786073
• NCBI GeneID: 5747
• Molecular Weight: 140.0 kDa
• NCBI Official Full Name: Homo sapiens PTK2 protein tyrosine kinase 2, mRNA
• NCBI Official Synonym Full Names: protein tyrosine kinase 2
• NCBI Official Symbol: PTK2
• NCBI Official Synonym Symbols: FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• NCBI Protein Information: focal adhesion kinase 1

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]