Western Blot (WB)
(NCKIPSD polyclonal antibody. Western Blot analysis of NCKIPSD expression in rat brain.)
Mouse anti-Human, Rat NCKIPSD Polyclonal Antibody | anti-NCKIPSD antibody
NCKIPSD (NCK Interacting Protein with SH3 Domain, AF3P21, 54kD VacA-interacting Protein, 54kD Vimentin-interacting Protein, VIP54, 90kD SH3 Protein Interacting with Nck, AF3p21, Dia-interacting Protein 1, DIP-1, Diaphanous Protein-interacting Protein, SH3
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(NCKIPSD polyclonal antibody. Western Blot analysis of NCKIPSD expression in rat brain.)
Western Blot (WB)
(NCKIPSD polyclonal antibody. Western Blot analysis of NCKIPSD expression in rat brain.)
Western Blot (WB)
(NCKIPSD polyclonal antibody. Western Blot analysis of NCKIPSD expression in HeLa.)
Western Blot (WB)
(NCKIPSD polyclonal antibody. Western Blot analysis of NCKIPSD expression in HeLa.)
Western Blot (WB)
(Western Blot analysis of NCKIPSD expression in transfected 293T cell line by NCKIPSD polyclonal antibody. Lane 1: NCKIPSD transfected lysate (78.65kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NCKIPSD expression in transfected 293T cell line by NCKIPSD polyclonal antibody. Lane 1: NCKIPSD transfected lysate (78.65kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Uniprot Description
Function: Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo
By similarity. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42
By similarity. May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis. Ref.13
Subunit structure: Associates with the intermediate filaments, vimentin and desmin. Binds the first and third SH3 domains of NCK. Binds the proline-rich domains of N-WASP through its SH3 domain
By similarity. Similarly, binds diaphanous protein homolog 1 (DRF1). Binds the SH3 domains of GRB2 through its proline-rich domains. Interacts with Helicobacter pylori toxin vacA. Isoform 4 interacts with FHOD1. Interacts with FASLG. Interacts with TMIGD2. Ref.2 Ref.6 Ref.11 Ref.13
Subcellular location: Nucleus. Note: Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.
Tissue specificity: Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen. Ref.2
Involvement in disease: A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with KMT2A/MLL1.
Sequence similarities: Contains 1 SH3 domain.
Sequence caution: The sequence BAG57476.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on NCKIPSD
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Product Notes
The NCKIPSD nckipsd (Catalog #AAA646745) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NCKIPSD (NCK Interacting Protein with SH3 Domain, AF3P21, 54kD VacA-interacting Protein, 54kD Vimentin-interacting Protein, VIP54, 90kD SH3 Protein Interacting with Nck, AF3p21, Dia-interacting Protein 1, DIP-1, Diaphanous Protein-interacting Protein, SH3 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCKIPSD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the NCKIPSD nckipsd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MYRALYAFRS AEPNALAFAA GETFLVLERS SAHWWLAARA RSGETGYVPP AYLRRLQGLE QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR KETLSRRGPS ASSVAVMTSS TSDHHLDAAA ARQPNGVCRA GFERQHSLPS SEHLGADGGL YQIPPQPRRA APTTPPPPVK RRDREALMAS GSGGHNTMPS GGNSVSSGSS VSSTSLDTLY TSSSPSEPGS SCSPTPPPVP RRGTHTTVSQ VQPPPSKASA PEPPAEEEVA TGTTSASDDL EALGTLSLGT TEEKAAAEAA VPRTIGAELM ELVRRNTGLS HELCRVAIGI IVGHIQASVP ASSPVMEQVL LSLVEGKDLS MALPSGQVCH DQQRLEVIFA DLARRKDDAQ QRSWALYEDE GVIRCYLEEL LHILTDADPE VCKKMCKRNE FESVLALVAY YQMEHRASLR LLLLKCFGAM CSLDAAIIST LVSSVLPVEL ARDMQTDTQD HQKLCYSALI LAMVFSMGEA VPYAHYEHLG TPFAQFLLNI VEDGLPLDTT EQLPDLCVNL LLALNLHLPA ADQNVIMAAL SKHANVKIFS EKLLLLLNRG DDPVRIFKHE PQPPHSVLKF LQDVFGSPAT AAIFYHTDMM ALIDITVRHI ADLSPGDKLR MEYLSLMHAI VRTTPYLQHR HRLPDLQAIL RRILNEEETS PQCQMDRMIV REMCKEFLVL GEAPS. It is sometimes possible for the material contained within the vial of "NCKIPSD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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