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Testing Data (FLUORESCENCE PROPERTIES OF FLICA 660POLY CASPASE REAGENT:Excitation max:660 nm Emission max: 690nm)

FLICA 660 Caspase 3/7 Assay Kit | FLICA assay kit

FLICA 660 Caspase 3/7 Assay Kit

Synonyms
FLICA 660 Caspase 3/7; FLICA 660 Caspase 3/7 Assay Kit; FLICA assay kit
Ordering
For Research Use Only!
Samples
Plasma Samples
Preparation and Storage
Store at 2-8 degree C

Testing Data

(FLUORESCENCE PROPERTIES OF FLICA 660POLY CASPASE REAGENT:Excitation max:660 nm Emission max: 690nm)

Testing Data (FLUORESCENCE PROPERTIES OF FLICA 660POLY CASPASE REAGENT:Excitation max:660 nm Emission max: 690nm)

Testing Data #2

(INTRACELLULAR APOPTOSIS DETECTION VIAACTIVE CASPASE-3 ASSAY To demonstrate the fluorescence properties andapoptosis detection capability of the new far-redfluorescent FLICA 660 Caspase-3 Assay, Jurkat cellswere were treated with 1 uM staurosporine for 4 hoursand labeled with FLICA 660-DEVD-FMK caspase-3inhibitor reagent. Using a Nikon E800 HyperspectralMicroscope equipped with aPhotometrics HQ2 camera, far-redfluorescence emission from apoptoticJurkat cells was acquired with a Cy5 HYQ bandpass filter combination set. FLICA660-DEVD-FMK reagent optimally excitesat 660 nm and has an emission max at690 nm, making it suitable for use withred lasers on common benchtop flowcytometers and ideal for dual stainingpurposes with green fluorescent probes. At left: Montage of DIC image andfluorescence image shows placement of Jurkat cells in sample and far-redemission from apoptosis-positive Jurkat cells stained with FLICA 660 Caspase-3Assay)

Testing Data #2 (INTRACELLULAR APOPTOSIS DETECTION VIAACTIVE CASPASE-3 ASSAY To demonstrate the fluorescence properties andapoptosis detection capability of the new far-redfluorescent FLICA 660 Caspase-3 Assay, Jurkat cellswere were treated with 1 uM staurosporine for 4 hoursand labeled with FLICA 660-DEVD-FMK caspase-3inhibitor reagent. Using a Nikon E800 HyperspectralMicroscope equipped with aPhotometrics HQ2 camera, far-redfluorescence emission from apoptoticJurkat cells was acquired with a Cy5 HYQ bandpass filter combination set. FLICA660-DEVD-FMK reagent optimally excitesat 660 nm and has an emission max at690 nm, making it suitable for use withred lasers on common benchtop flowcytometers and ideal for dual stainingpurposes with green fluorescent probes. At left: Montage of DIC image andfluorescence image shows placement of Jurkat cells in sample and far-redemission from apoptosis-positive Jurkat cells stained with FLICA 660 Caspase-3Assay)
Related Product Information for FLICA assay kit
Background/Introduction: Factor XII or Hageman factor (EC 3.4.21.38), is the zymogen form of factor XIIa, a serine protease involved in Coagulation Pathway. This single chain zymogen is activated by Kallikrein into a two-chain serine protease (XIIa) with a heavy chain (factor XIIa-alpha) and a light chain. Human Factor XII and Prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates Prekallikrein to Kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. The defects in this gene do not cause any clinical symptoms but prolong the whole blood clotting time. Factor XIIa activity assay kit utilizes the ability of factor XIIa to cleave a synthetic substrate to release ????-Nitroaniline (????NA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma samples.
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Product Notes

The FLICA (Catalog #AAA258001) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "FLICA 660 Caspase 3/7, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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