Rabbit anti-Mouse BID Polyclonal Antibody | anti-BID antibody

BID (Ser61) (BH3-interacting Domain Death Agonist, p22 BID) (Biotin)

Cat. Num. AAA6465309

• Gene Names: Bid; AI875481; AU022477; 2700049M22Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: BID; Polyclonal Antibody; BID (Ser61) (BH3-interacting Domain Death Agonist; p22 BID) (Biotin); anti-BID antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse BID.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BID antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic non-phosphopeptide corresponding to amino acid residues surrounding Ser61 of mouse BID (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-mouse BID Antibody (S61) in mouse spleen tissue lysates (35ug/lane). BID (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(mouse BID Antibody (S61) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(mouse BID Antibody (S61) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-BID antibody

Antibodies; Apoptosis

References

1. Ziporen, L., J. Immunol. 182(1), 515-521 (2009). 2. Kaufmann, T., Immunity 30(1), 56-66 (2009). 3. Mandal, M., Proc. Natl. Acad. Sci. USA 105(52), 20840-20845 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 31542228
• NCBI GeneID: 12122
• NCBI Accession #: NP_031570
• NCBI GenBank Nucleotide #: NM_007544.3
• UniProt Accession #: P70444; Q99M39
• Molecular Weight: 21,952 Da
• NCBI Official Full Name: BH3-interacting domain death agonist
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: Bid
• NCBI Official Synonym Symbols: AI875481; AU022477; 2700049M22Rik
• NCBI Protein Information: BH3-interacting domain death agonist
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: Bidirectional sugar transporter
• UniProt Gene Name: Bid
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_MOUSE

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Apoptosis; Mitochondrial

Cellular Component: membrane; mitochondrion; cell; cytoplasm; intracellular; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; positive regulation of apoptosis; apoptosis; DNA damage response, signal transduction; regulation of protein oligomerization; regulation of cell proliferation; regulation of apoptosis; positive regulation of protein oligomerization; apoptotic mitochondrial changes; protein targeting to mitochondrion; protein homooligomerization