Rabbit anti-Mouse Bid Polyclonal Antibody | anti-Bid antibody

Bid Antibody

Cat. Num. AAA840806

• Gene Names: Bid; AI875481; AU022477; 2700049M22Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Bid; Polyclonal Antibody; Bid Antibody; FP497; MGC42355; MGC15319; anti-Bid antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 195

• Applicable Applications for anti-Bid antibody: Western Blot (WB)
• Application Notes: Western blot analysis (0.5-5 ug/ml) also works well in immunoprecipitation (20 ug/ml) and Immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the full length (22 kDa) mouse Bid. Reactivity to other species has not been tested.
• Immunogen: Synthetic peptide surrounding amino acid 45 of mouse Bid
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Bid antibody

Background: Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed thromugh Fas.

NCBI and Uniprot Product Information

• NCBI GI #: 31542228
• NCBI GeneID: 12122
• NCBI Accession #: NP_031570.2
• NCBI GenBank Nucleotide #: NM_007544.3
• UniProt Accession #: P70444; Q99M39
• Molecular Weight: 21,952 Da
• NCBI Official Full Name: BH3-interacting domain death agonist
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: Bid
• NCBI Official Synonym Symbols: AI875481; AU022477; 2700049M22Rik
• NCBI Protein Information: BH3-interacting domain death agonist
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: Bidirectional sugar transporter
• UniProt Gene Name: Bid
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_MOUSE

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Apoptosis; Mitochondrial

Cellular Component: membrane; mitochondrion; cell; cytoplasm; intracellular; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: caspase activation; regulation of apoptosis; positive regulation of protein oligomerization; apoptotic mitochondrial changes; release of cytochrome c from mitochondria; positive regulation of apoptosis; positive regulation of protein homooligomerization; apoptosis; DNA damage response, signal transduction; regulation of protein oligomerization; protein targeting to mitochondrion; protein homooligomerization; regulation of cell proliferation