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Western Blot (WB)
(Anti-SH3KBP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: THP1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:73 kDa Observed band size:80 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human SH3KBP1 Polyclonal Antibody | anti-SH3KBP1 antibody
Anti-SH3KBP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
SH3KBP1; Polyclonal Antibody; Anti-SH3KBP1 Antibody; Rabbit Polyclonal; SH3KBP1 Antibody; Rabbit PAb; Antigen Affinity Purified; SH3-domain kinase binding protein 1; Anti-CD2BP3 Antibody; Anti-CIN85 Antibody; Anti-GIG10 Antibody; Anti-HSB-1 Antibody; Anti-HSB1 Antibody; Anti-MIG18 Antibody; anti-SH3KBP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SH3KBP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SH3KBP1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
IP: 1-5uL/mg of lysate
IHC-P: 1:200-1:1000
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human SH3KBP1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SH3KBP1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-SH3KBP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: THP1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:73 kDa Observed band size:80 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-SH3KBP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: THP1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:73 kDa Observed band size:80 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human SH3KBP1 in human lymph node with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human SH3KBP1 in human lymph node with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human SH3KBP1 in human placenta with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human SH3KBP1 in human placenta with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(SH3KBP1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-SH3KBP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SH3KBP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 73 kDa Observed band size :80 kDa)
Immunoprecipitation (IP)
(SH3KBP1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-SH3KBP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SH3KBP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 73 kDa Observed band size :80 kDa)
Related Product Information for anti-SH3KBP1 antibody
SH3KBP1 (SH3 Domain Containing Kinase Binding Protein 1, also known as CIN85) is a Protein Coding gene. The SH3KBP1 gene, located on Xp22.12, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline-rich region, and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion, and the regulation of clathrin-dependent endocytosis. SH3KBP1 is widely expressed in the lymph node, fat, and other tissues. Diseases associated with SH3KBP1 include Immunodeficiency 61 and Adrenal Cortical Adenocarcinoma. SH3KBP1 is a novel target of SOX10.
References
Moncalian G, et al. (2006) Atypical polyproline recognition by the cms n-terminal src homology 3 domain. J Biol Chem 281 (50): 38845-38853.Hodonsky CJ, et al. (2012) Sox10 regulates expression of the sh3-domain kinase binding protein 1 (sh3kbp1) locus in schwann cells via an alternative promoter. Mol Cell Neurosci 49 (2): 85-96.Diesenberg K, et al. (2015) Sept9 negatively regulates ubiquitin-dependent downregulation of egfr. J Cell Sci 128 (2): 397-407.
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Product Notes
The SH3KBP1 (Catalog #AAA8108164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SH3KBP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SH3KBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:200-1:1000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the SH3KBP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH3KBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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