Mouse anti-Human MD-2, Soluble Monoclonal Antibody | anti-LY96 antibody

MD-2, Soluble (TLR4/sMD-2 Complex, Toll-like Receptor 4, hToll, CD284, ARMD10/Lymphocyte Antigen 96, Ly-96, ESOP-1, Soluble Protein MD-2, LY96, ESOP1)

Cat. Num. AAA646441

• Gene Names: LY96; MD2; MD-2; ly-96; ESOP-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MD-2; Soluble; Monoclonal Antibody; Soluble (TLR4/sMD-2 Complex; Toll-like Receptor 4; hToll; CD284; ARMD10/Lymphocyte Antigen 96; Ly-96; ESOP-1; Soluble Protein MD-2; LY96; ESOP1); Anti -MD-2; anti-LY96 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 13A56
• Specificity: Recognizes both the monomeric and the polymeric form of human sMD-2. It inhibits LPS binding to MD-2. It does not cross-react with mouse MD-2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA.

• Applicable Applications for anti-LY96 antibody: ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunofluorescence.; Dilution: Western Blot: 1:50; ELISA: 1:50; Immunofluorescence: 1:50
• Immunogen: Recombinant corresponding to human MD-2, His-tagged, expressed in Baculovirus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-LY96 antibody

TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomer and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells. This monoclonal antibody reacts with both the monomeric and the polymeric form of sMD-2. In addition, it is able to inhibit LPS binding to MD-2.

Product Categories/Family for anti-LY96 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 5051740
• NCBI GeneID: 23643
• UniProt Accession #: Q9Y6Y9; B3Y6A5
• Molecular Weight: 18,546 Da
• NCBI Official Full Name: MD-2
• NCBI Official Synonym Full Names: lymphocyte antigen 96
• NCBI Official Symbol: LY96
• NCBI Official Synonym Symbols: MD2; MD-2; ly-96; ESOP-1
• NCBI Protein Information: lymphocyte antigen 96; protein MD-2; myeloid differentiation protein-2
• UniProt Protein Name: Lymphocyte antigen 96
• Protein Family: Lymphocyte antigen
• UniProt Gene Name: LY96
• UniProt Synonym Gene Names: ESOP1; MD2; Ly-96
• UniProt Entry Name: LY96_HUMAN

NCBI Description

This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2010]

Uniprot Description

Function: Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.

Subunit structure: Heterogeneous homopolymer formed from homodimers; disulfide-linked. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binds to the extracellular domains of TLR2 and TLR4. Ligand binding induces interaction with TLR4 and oligomerization of the complex. Ref.7 Ref.8 Ref.9 Ref.11

Subcellular location: Secreted › extracellular space.

Post-translational modification: N-glycosylated; high-mannose. Ref.8 Ref.11