Rabbit anti-Human GPC4 Polyclonal Antibody | anti-GPC4 antibody

GPC4, ID (Glypican-4, K-glypican, Secreted Glypican-4, UNQ474/PRO937) (Biotin)

Cat. Num. AAA6478618

• Gene Names: GPC4; K-glypican
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GPC4; Polyclonal Antibody; ID (Glypican-4; K-glypican; Secreted Glypican-4; UNQ474/PRO937) (Biotin); anti-GPC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GPC4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GPC4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human GPC4 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GPC4 Antibody (Center) in K562 cell line lysates (35ug/lane). GPC4 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GPC4 Antibody (Center) in 293 cell line lysates (35ug/lane). GPC4 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-GPC4 antibody

Antibodies; Proteoglycans

References

1. Karumanchi, S.A., Mol. Cell 7(4), 811-822 (2001). 2. Hagihara, K., Dev. Dyn. 219(3), 353-367 (2000). 3. Veugelers, M., Hum. Mol. Genet. 9(9), 1321-1328 (2000). 4. Veugelers, M., Genomics 53(1), 1-11 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 21614525
• NCBI GeneID: 2239
• NCBI Accession #: NP_001439
• NCBI GenBank Nucleotide #: NM_001448.2
• UniProt Accession #: O75487; Q6ZMA6; Q96L43; Q9NU08; Q9UJN1; Q9UPD9; B2R6J7; B4E2C0
• Molecular Weight: 54,910 Da
• NCBI Official Full Name: glypican-4
• NCBI Official Synonym Full Names: glypican 4
• NCBI Official Symbol: GPC4
• NCBI Official Synonym Symbols: K-glypican
• NCBI Protein Information: glypican-4; dJ900E8.1 (glypican 4); glypican proteoglycan 4
• UniProt Protein Name: Glypican-4
• Protein Family: Glypican
• UniProt Gene Name: GPC4
• UniProt Entry Name: GPC4_HUMAN

Uniprot Description

GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: proteinaceous extracellular matrix; extracellular space; lysosomal lumen; integral to plasma membrane; Golgi lumen; plasma membrane; nucleus; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; phototransduction, visible light; cell proliferation; glycosaminoglycan catabolic process; anatomical structure morphogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1