Rabbit anti-Human ATP5O Polyclonal Antibody | anti-ATP5O antibody

ATP5O, NT (ATP Synthase Subunit O, Mitochondrial, Oligomycin Sensitivity Conferral Protein, OSCP, ATP5O) (Azide free) (HRP)

Cat. Num. AAA6464379

• Gene Names: ATP5O; ATPO; OSCP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ATP5O; Polyclonal Antibody; NT (ATP Synthase Subunit O; Mitochondrial; Oligomycin Sensitivity Conferral Protein; OSCP; ATP5O) (Azide free) (HRP); anti-ATP5O antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP5O.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ATP5O antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ATP5O (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP5O Antibody (N-term) in 293 cell line lysates (35ug/lane). ATP5O (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ATP5O antibody

Antibodies; Enzymes; Synthase

References

1. Wang, L., et.al., Cancer Epidemiol. Biomarkers Prev. 17(12), 3558-3566 (2008). 2. Contessi, S., et.al., J. Bioenerg. Biomembr. 39(4), 291-300 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4502303
• NCBI GeneID: 539
• NCBI Accession #: NP_001688.1
• NCBI GenBank Nucleotide #: NM_001697.2
• UniProt Accession #: P48047; Q5U042; Q6IBI2; B2R4E2
• Molecular Weight: 23,277 Da
• NCBI Official Full Name: ATP synthase subunit O, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
• NCBI Official Symbol: ATP5O
• NCBI Official Synonym Symbols: ATPO; OSCP
• NCBI Protein Information: ATP synthase subunit O, mitochondrial; human ATP synthase OSCP subunit; oligomycin sensitivity conferral protein; oligomycin sensitivity conferring protein
• UniProt Protein Name: ATP synthase subunit O, mitochondrial
• UniProt Gene Name: ATP5O
• UniProt Synonym Gene Names: ATPO; OSCP
• UniProt Entry Name: ATPO_HUMAN

NCBI Description

The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5O: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase delta chain family.

Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Hydrolase

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: mitochondrion; mitochondrial inner membrane; plasma membrane; nucleus; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; drug binding; transmembrane transporter activity

Biological Process: cellular metabolic process; proton transport; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport