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Western Blot (WB)
((0. 001ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TPPP/p25 Polyclonal Antibody | anti-TPPP antibody
Goat anti-TPPP/p25 Antibody
Gene Names
TPPP; p24; p25; TPPP1; TPPP/p25; p25alpha
Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TPPP/p25; Polyclonal Antibody; Goat anti-TPPP/p25 Antibody; brain specific protein p25 alpha; glycogen synthase kinase 3 (GSK3) inhibitor p24; p24; p25; p25alpha; TPPP1; tubulin polymerization promoting protein; tubulin polymerization-promoting protein; TPPP; anti-TPPP antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DPSKDRAAKRLSLES
Sequence Length
219
Applicable Applications for anti-TPPP antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2ug/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75ug/ml.
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2ug/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75ug/ml.
Immunogen
Peptide with sequence C-DPSKDRAAKRLSLES, from the internal region of the protein sequence according to NP_008961.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 001ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 001ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((0. 001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
Immunohistochemistry
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
Immunohistochemistry
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,694 Da
NCBI Official Full Name
tubulin polymerization-promoting protein
NCBI Official Synonym Full Names
tubulin polymerization promoting protein
NCBI Official Symbol
TPPP
NCBI Official Synonym Symbols
p24; p25; TPPP1; TPPP/p25; p25alpha
NCBI Protein Information
tubulin polymerization-promoting protein
UniProt Protein Name
Tubulin polymerization-promoting protein
Protein Family
UniProt Gene Name
TPPP
UniProt Synonym Gene Names
TPPP1; TPPP
UniProt Entry Name
TPPP_HUMAN
Uniprot Description
TPPP: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. Belongs to the TPPP family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 5p15.3
Cellular Component: cytoplasm; microtubule; myelin sheath; nucleus; perinuclear region of cytoplasm
Molecular Function: microtubule binding; protein binding; tubulin binding
Biological Process: microtubule bundle formation; microtubule polymerization; positive regulation of protein complex assembly; positive regulation of protein polymerization
Research Articles on TPPP
Similar Products
Product Notes
The TPPP tppp (Catalog #AAA422281) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TPPP/p25 Antibody reacts with Tested: Human, Mouse, Rat Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPPP/p25 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2ug/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75ug/ml. Researchers should empirically determine the suitability of the TPPP tppp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DPSKDRAAKR LSLES. It is sometimes possible for the material contained within the vial of "TPPP/p25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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