Western Blot (WB)
(Western Blot analysis using MBS646213 in A549 cell line lysates (35ug/lane).This demonstrates the CLU antibody detected the CLU protein (arrow).)
Rabbit anti-Human CLU Polyclonal Antibody | anti-CLU antibody
CLU, NT (CLU, APOJ, CLI, KUB1, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, Clusterin beta
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western Blot analysis using MBS646213 in A549 cell line lysates (35ug/lane).This demonstrates the CLU antibody detected the CLU protein (arrow).)
Western Blot (WB)
(Western Blot analysis using MBS646213 in A549 cell line lysates (35ug/lane).This demonstrates the CLU antibody detected the CLU protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS646213 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646213for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS646213 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646213for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A549 cells (right histogram) using MBS646213compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A549 cells (right histogram) using MBS646213compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
Uniprot Description
Function: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.33 Ref.37 Ref.38 Ref.40 Ref.42 Ref.46 Ref.47 Ref.50 Ref.51 Ref.52
Subunit structure: Antiparallel disulfide-linked heterodimer of an alpha chain and a beta chain. Self-associates and forms higher oligomers. Interacts with a broad range of misfolded proteins, including APP, APOC2 and LYZ. Slightly acidic pH promotes interaction with misfolded proteins. Forms high-molecular weight oligomers upon interaction with misfolded proteins. Interacts with APOA1, LRP2, CLUAP1 AND PON1. Interacts with the complement complex. Interacts (via alpha chain) with XRCC6. Interacts with SYVN1, COMMD1, BTRC, CUL1 and with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes. Interacts (via alpha chain) with BAX in stressed cells, where BAX undergoes a conformation change leading to association with the mitochondrial membrane. Does not interact with BAX in unstressed cells. Found in a complex with LTF, CLU, EPPIN and SEMG1. Ref.1 Ref.9 Ref.10 Ref.11 Ref.14 Ref.18 Ref.20 Ref.21 Ref.22 Ref.25 Ref.26 Ref.27 Ref.30 Ref.33 Ref.37 Ref.39 Ref.40 Ref.42 Ref.44 Ref.46 Ref.51
Subcellular location: Isoform 1: Secreted. Note: Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Ref.1 Ref.10 Ref.11 Ref.13 Ref.22 Ref.24 Ref.27 Ref.33 Ref.37 Ref.38 Ref.40 Ref.44 Ref.47 Ref.51Nucleus. Cytoplasm. Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle › secretory vesicle › chromaffin granule
By similarity. Note: Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. Ref.1 Ref.10 Ref.11 Ref.13 Ref.22 Ref.24 Ref.27 Ref.33 Ref.37 Ref.38 Ref.40 Ref.44 Ref.47 Ref.51
Tissue specificity: Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. Ref.1 Ref.2 Ref.10 Ref.11 Ref.13 Ref.14 Ref.22 Ref.24 Ref.37 Ref.40 Ref.43
Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). Up-regulated by agents that induce apoptosis, both at mRNA and protein level. Isoform 1 is up-regulated by androgen. Isoform 2 is down-regulated by androgen. Ref.34 Ref.38 Ref.41
Post-translational modification: Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Ref.10Polyubiquitinated, leading to proteasomal degradation.Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. Ref.10 Ref.18 Ref.23 Ref.29 Ref.37
Sequence similarities: Belongs to the clusterin family.
Sequence caution: The sequence AAA35692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAB06508.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAB06508.1 differs from that shown. Reason: Contaminating sequence.The sequence AAH10514.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH19588.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAP88927.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAP88927.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAT08041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAG36598.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAA32847.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on CLU
Similar Products
Product Notes
The CLU clu (Catalog #AAA646213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLU, NT (CLU, APOJ, CLI, KUB1, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, Clusterin beta reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLU can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CLU clu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
