Western Blot (WB)
(Staining (0.3ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat XAB2/HCNP Polyclonal Antibody | anti-XAB2 antibody
Goat anti-XAB2/HCNP Antibody
Gene Names
XAB2; HCNP; HCRN; SYF1; NTC90
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
XAB2/HCNP; Polyclonal Antibody; Goat anti-XAB2/HCNP Antibody; XAB2; HCNP; XPA binding protein 2; HCRN; crn-related protein kim1; DKFZp762C1015; NTC90; SYF1; SYF1 homolog; RNA splicing factor; XAB2 antibody; HCNP antibody; XPA binding protein 2 antibody; HCRN antibody; crn-related protein kim1 antibody; DKFZp762C1015 antibody; NTC90 antibody; SYF1 antibody; RNA splicing factor antibody; XAB2 / HCNP; anti-XAB2 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SVPAAVFGSLKED
Sequence Length
855
Applicable Applications for anti-XAB2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows nuclear staining. Recommended concentration, 3-4ug/ml.
Western Blot: Approx 90kDa band observed in human fibroblast A431 lysates (calculated MW of 100kDa according to NP_064581.2). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows nuclear staining. Recommended concentration, 3-4ug/ml.
Western Blot: Approx 90kDa band observed in human fibroblast A431 lysates (calculated MW of 100kDa according to NP_064581.2). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-SVPAAVFGSLKED, from the C Terminus of the protein sequence according to NP_064581.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Staining (0.3ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.3ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100,010 Da
NCBI Official Full Name
pre-mRNA-splicing factor SYF1
NCBI Official Synonym Full Names
XPA binding protein 2
NCBI Official Symbol
XAB2
NCBI Official Synonym Symbols
HCNP; HCRN; SYF1; NTC90
NCBI Protein Information
pre-mRNA-splicing factor SYF1
UniProt Protein Name
Pre-mRNA-splicing factor SYF1
UniProt Gene Name
XAB2
UniProt Synonym Gene Names
HCNP; KIAA1177; SYF1
UniProt Entry Name
SYF1_HUMAN
Uniprot Description
XAB2: Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing. Belongs to the crooked-neck family.
Protein type: Spliceosome; RNA splicing
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: membrane; nucleoplasm; nucleus
Molecular Function: protein binding
Biological Process: blastocyst development; cerebral cortex development; generation of catalytic spliceosome for first transesterification step; nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; transcription-coupled nucleotide-excision repair
Research Articles on XAB2
Similar Products
Product Notes
The XAB2 xab2 (Catalog #AAA420010) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-XAB2/HCNP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's XAB2/HCNP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows nuclear staining. Recommended concentration, 3-4ug/ml. Western Blot: Approx 90kDa band observed in human fibroblast A431 lysates (calculated MW of 100kDa according to NP_064581.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the XAB2 xab2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SVPAAVFGSL KED. It is sometimes possible for the material contained within the vial of "XAB2/HCNP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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