Rabbit ALAS2 Polyclonal Antibody | anti-ALAS2 antibody

ALAS2 Polyclonal Antibody

Cat. Num. AAA3007833

• Gene Names: ALAS2; ASB; ANH1; XLSA; ALASE; XLDPP; XLEPP; ALAS-E; SIDBA1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ALAS2; Polyclonal Antibody; ALAS2 Polyclonal Antibody; 5-aminolevulinate synthase; erythroid-specific; mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2; ALASE; ASB; anti-ALAS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ALAS2 polyclonal antibody detects endogenous levels of ALAS2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 587

• Applicable Applications for anti-ALAS2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:10-1:100
• Immunogen: Recombinant full length Human ALAS2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ALAS2 polyclonal antibody)

Related Product Information for anti-ALAS2 antibody

5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

NCBI and Uniprot Product Information

• NCBI GI #: 83977442
• NCBI GeneID: 212
• NCBI Accession #: NP_000023.2
• NCBI GenBank Nucleotide #: NP_000023.2
• UniProt Accession #: P22557
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: 5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a
• NCBI Official Synonym Full Names: 5'-aminolevulinate synthase 2
• NCBI Official Symbol: ALAS2
• NCBI Official Synonym Symbols: ASB; ANH1; XLSA; ALASE; XLDPP; XLEPP; ALAS-E; SIDBA1
• NCBI Protein Information: 5-aminolevulinate synthase, erythroid-specific, mitochondrial
• UniProt Protein Name: 5-aminolevulinate synthase, erythroid-specific, mitochondrial
• Protein Family: Alanine--tRNA ligase
• UniProt Gene Name: ALAS2
• UniProt Synonym Gene Names: ALASE; ASB; ALAS-E
• UniProt Entry Name: HEM0_HUMAN

NCBI Description

The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]