Rabbit anti-Human, Mouse ACOX1 Polyclonal Antibody | anti-ACOX1 antibody

ACOX1, NT (Peroxisomal Acyl-coenzyme A Oxidase 1, Acyl-coenzyme A Oxidase 1, Peroxisomal, Palmitoyl-CoA Oxidase, AOX, Straight-chain Acyl-CoA Oxidase, SCOX, ACOX) (FITC)

Cat. Num. AAA6461139

• Gene Names: ACOX1; ACOX; SCOX; PALMCOX
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ACOX1; Polyclonal Antibody; NT (Peroxisomal Acyl-coenzyme A Oxidase 1; Acyl-coenzyme A Oxidase 1; Peroxisomal; Palmitoyl-CoA Oxidase; AOX; Straight-chain Acyl-CoA Oxidase; SCOX; ACOX) (FITC); anti-ACOX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACOX1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 660

• Applicable Applications for anti-ACOX1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:1,000; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ACOX1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(ACOX1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACOX1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ACOX1 antibody

Antibodies; Enzymes; Oxidase

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Chu, R., et al., J. Biol. Chem. 270(9):4908-4915 (1995). 3. Aoyama, T., et al., Biochem. Biophys. Res. Commun. 198(3):1113-1118 (1994). 4. Fournier, B., et al., J. Clin. Invest. 94(2):526-531 (1994). 5. Varanasi, U., et al., Proc. Natl. Acad. Sci. USA 91(8):3107-3111 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 30089974
• NCBI GeneID: 51
• NCBI Accession #: NP_009223
• NCBI GenBank Nucleotide #: NP_009223.2
• UniProt Accession #: Q15067
• Molecular Weight: 74.442
• NCBI Official Full Name: peroxisomal acyl-coenzyme A oxidase 1 isoform b
• NCBI Official Synonym Full Names: acyl-CoA oxidase 1
• NCBI Official Symbol: ACOX1
• NCBI Official Synonym Symbols: ACOX; SCOX; PALMCOX
• NCBI Protein Information: peroxisomal acyl-coenzyme A oxidase 1
• UniProt Protein Name: Peroxisomal acyl-coenzyme A oxidase 1
• Protein Family: Peroxisomal acyl-coenzyme A oxidase
• UniProt Gene Name: ACOX1
• UniProt Synonym Gene Names: AOX1 PublicationManual assertion based on opinion iniRef.4; SCOX

NCBI Description

The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.