Rabbit anti-Human ACOX1 Polyclonal Antibody | anti-ACOX1 antibody

ACOX1, CT (Peroxisomal Acyl-coenzyme A Oxidase 1, Acyl-coenzyme A Oxidase 1, Peroxisomal, Palmitoyl-CoA Oxidase, AOX, Straight-chain Acyl-CoA Oxidase, SCOX, ACOX) (Azide free) (HRP)

Cat. Num. AAA6461129

• Gene Names: ACOX1; ACOX; SCOX; PALMCOX
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ACOX1; Polyclonal Antibody; CT (Peroxisomal Acyl-coenzyme A Oxidase 1; Acyl-coenzyme A Oxidase 1; Peroxisomal; Palmitoyl-CoA Oxidase; AOX; Straight-chain Acyl-CoA Oxidase; SCOX; ACOX) (Azide free) (HRP); anti-ACOX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACOX1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 660

• Applicable Applications for anti-ACOX1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ACOX1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (RB04651). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (RB04652). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-ACOX1 antibody

Antibodies; Enzymes; Oxidase

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Chu, R., et al., J. Biol. Chem. 270(9):4908-4915 (1995). 3. Aoyama, T., et al., Biochem. Biophys. Res. Commun. 198(3):1113-1118 (1994). 4. Fournier, B., et al., J. Clin. Invest. 94(2):526-531 (1994). 5. Varanasi, U., et al., Proc. Natl. Acad. Sci. USA 91(8):3107-3111 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 30089974
• NCBI GeneID: 51
• NCBI Accession #: NP_009223
• NCBI GenBank Nucleotide #: NP_009223.2
• UniProt Accession #: Q15067
• Molecular Weight: 74.442
• NCBI Official Full Name: peroxisomal acyl-coenzyme A oxidase 1 isoform b
• NCBI Official Synonym Full Names: acyl-CoA oxidase 1
• NCBI Official Symbol: ACOX1
• NCBI Official Synonym Symbols: ACOX; SCOX; PALMCOX
• NCBI Protein Information: peroxisomal acyl-coenzyme A oxidase 1
• UniProt Protein Name: Peroxisomal acyl-coenzyme A oxidase 1
• Protein Family: Peroxisomal acyl-coenzyme A oxidase
• UniProt Gene Name: ACOX1
• UniProt Synonym Gene Names: AOX1 PublicationManual assertion based on opinion iniRef.4; SCOX

NCBI Description

The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.