Western Blot (WB)
(Anti-Glutaredoxin 2 antibody, MBS176047, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: U87 Cell LysateLane 4: NEU Cell LysateLane 5: JURKAT Cell LysateLane 6: MCF-7 Cell Lysate )
Rabbit Glutaredoxin 2 Polyclonal Antibody | anti-GLRX2 antibody
Anti-Glutaredoxin 2 antibody
Concentration: 0.1-0.5 ug/mL
Tested Species: Hu, Rat
Predicted Species: Ms
Immunohistochemistry (Paraffin-embedded Section)
Concentration: 0.5-1 ug/mL
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Tested Species: In- house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Glutaredoxin 2 antibody, MBS176047, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: U87 Cell LysateLane 4: NEU Cell LysateLane 5: JURKAT Cell LysateLane 6: MCF-7 Cell Lysate )
Western Blot (WB)
(Anti-Glutaredoxin 2 antibody, MBS176047, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: U87 Cell LysateLane 4: NEU Cell LysateLane 5: JURKAT Cell LysateLane 6: MCF-7 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-Glutaredoxin 2 antibody, MBS176047, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-Glutaredoxin 2 antibody, MBS176047, IHC(P)IHC(P): Rat Brain Tissue )
Background: GLRX2(Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins(e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al.(2001) and Gladyshev et al.(2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al.(2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon(exon 1B) located upstream from the first exon used by the GLRX2A transcript(exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al.(2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. [provided by RefSeq, Aug 2011]
Uniprot Description
GLRX2: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. Belongs to the glutaredoxin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; Mitochondrial
Chromosomal Location of Human Ortholog: 1q31.2
Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; nucleus
Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; arsenate reductase (glutaredoxin) activity; glutathione disulfide oxidoreductase activity
Biological Process: response to organic substance; response to temperature stimulus; DNA protection; glutathione metabolic process; response to hydrogen peroxide; regulation of transcription, DNA-dependent; protein folding; apoptosis; cell redox homeostasis; regulation of signal transduction; response to redox state; cell differentiation
Research Articles on GLRX2
Similar Products
Product Notes
The GLRX2 glrx2 (Catalog #AAA176047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Glutaredoxin 2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutaredoxin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western blot Concentration: 0.1-0.5 ug/mL Tested Species: Hu, Rat Predicted Species: Ms Immunohistochemistry (Paraffin-embedded Section) Concentration: 0.5-1 ug/mL Tested Species: Hu, Rat Predicted Species: Ms Antigen Retrieval: By Heat Tested Species: In- house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Researchers should empirically determine the suitability of the GLRX2 glrx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutaredoxin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
