Western Blot (WB)
(Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit ADROPIN Polyclonal Antibody | anti-ENHO antibody
ADROPIN Antibody
Gene Names
ENHO; UNQ470; C9orf165
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ADROPIN antibody is affinity chromatography purified via peptide column.
Synonyms
ADROPIN; Polyclonal Antibody; ADROPIN Antibody; UNQ470; C9orf165; UNQ470/PRO830; Adropin; Energy homeostasis-associated protein; anti-ENHO antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ADROPIN antibody is human, mouse and rat reactive. Despite its predicted molecular weight, ADROPIN often migrates at a higher than expected molecular weight in SDS-PAGE.
Purity/Purification
ADROPIN antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
76
Applicable Applications for anti-ENHO antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
Optimal dilutions to be determined by the researcher
Optimal dilutions to be determined by the researcher
Conjugate
Unconjugated
Immunogen
ADROPIN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ADROPIN.
The immunogen is located within the last 50 amino acids of ADROPIN
The immunogen is located within the last 50 amino acids of ADROPIN
Buffer
ADROPIN antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: 94%
Positive Control
MBS151732- Human Brain Tissue Lysate
MBS154323- Human Brain Tissue Slide
MBS154323- Human Brain Tissue Slide
Preparation and Storage
ADROPIN antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ADROPIN in human brain tissue with ADROPIN antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ADROPIN in human brain tissue with ADROPIN antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ADROPIN in human brain tissue with ADROPIN antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ADROPIN in human brain tissue with ADROPIN antibody at 20 μg/mL.)
Related Product Information for anti-ENHO antibody
ADROPIN is a recently identified protein that has been implicated in the maintenance of energy homeostasis and insulin resistance (1-3). ADROPIN expression is regulated by energy status and dietary nutrient content and is altered by obesity and regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma (PPAR-gamma) (1). ADROPIN levels increase with dietary fat content (2). ADROPIN has also been proposed to play a role in the regulation of endothelial function (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 8 kDa
Observed: 16 kDa
Observed: 16 kDa
NCBI Official Full Name
adropin
NCBI Official Synonym Full Names
energy homeostasis associated
NCBI Official Symbol
ENHO
NCBI Official Synonym Symbols
UNQ470; C9orf165
NCBI Protein Information
adropin; GAAI470; energy homeostasis-associated protein
UniProt Protein Name
Adropin
Protein Family
UniProt Gene Name
ENHO
UniProt Synonym Gene Names
C9orf165
UniProt Entry Name
ENHO_HUMAN
Uniprot Description
ENHO: Involved in the regulation of glucose homestasis and lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Membrane protein, integral; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: extracellular region
Research Articles on ENHO
Similar Products
Product Notes
The ENHO enho (Catalog #AAA153445) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADROPIN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADROPIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the ENHO enho for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADROPIN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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