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Immunohistochemistry (IHC) (Immunohistochemical staining of DLK1 MBS646035 in placenta, villi (1:500 dilution).|This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)

Mouse DLK1 Monoclonal Antibody | anti-Dlk1 antibody

DLK1 (Protein Delta Homolog 1, Fetal Antigen 1, Preadipocyte Factor 1, Pref-1, FA1)

Gene Names
Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
DLK1; Monoclonal Antibody; DLK1 (Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1); Anti -DLK1 (Protein Delta Homolog 1; anti-Dlk1 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
PF299-1
Specificity
Recognizes human DLK1. Does not cross-react with mouse DLK1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Applicable Applications for anti-Dlk1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Immunogen
Recombinant human DLK1 (extracellular domain).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical staining of DLK1 MBS646035 in placenta, villi (1:500 dilution).|This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)

Immunohistochemistry (IHC) (Immunohistochemical staining of DLK1 MBS646035 in placenta, villi (1:500 dilution).|This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)

Flow Cytometry (FC/FACS)

(FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using MBS646035)

Flow Cytometry (FC/FACS) (FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using MBS646035)

Western Blot (WB)

(Western blot analysis of human Pref-1using MBS646035 at 1: 2000 dilution:|A.|1. Human Pref-1 Fc protein.|2. Transfected human Pref-1 full length cell lysate (HEK 293).|3. Mock Transfected HEK293 cell lysate.|B.|1. Human Pref-1 Fc protein.|2. Mouse Pref-1 Fc protein.|3. Human DNER Fc protein.|4. Human DLL1 Fc protein.|5. Human DLL3 Fc protein.|6. Human DLL4 Fc protein.|7. Human Jagged1 Fc protein.|8. Human FGF23 Fc protein.)

Western Blot (WB) (Western blot analysis of human Pref-1using MBS646035 at 1: 2000 dilution:|A.|1. Human Pref-1 Fc protein.|2. Transfected human Pref-1 full length cell lysate (HEK 293).|3. Mock Transfected HEK293 cell lysate.|B.|1. Human Pref-1 Fc protein.|2. Mouse Pref-1 Fc protein.|3. Human DNER Fc protein.|4. Human DLL1 Fc protein.|5. Human DLL3 Fc protein.|6. Human DLL4 Fc protein.|7. Human Jagged1 Fc protein.|8. Human FGF23 Fc protein.)
Related Product Information for anti-Dlk1 antibody
DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.
Product Categories/Family for anti-Dlk1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,320 Da
NCBI Official Full Name
Dlk1
NCBI Official Synonym Full Names
delta-like 1 homolog (Drosophila)
NCBI Official Symbol
Dlk1
NCBI Official Synonym Symbols
FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
NCBI Protein Information
protein delta homolog 1; fetal antigen 1; preadipocyte factor 1; stromal cell derived protein 1; adipocyte differentiation inhibitor protein
UniProt Protein Name
Protein delta homolog 1
Protein Family
UniProt Gene Name
Dlk1
UniProt Synonym Gene Names
Dlk; Pref1; Scp-1; DLK-1; Pref-1; FA1
UniProt Entry Name
DLK1_MOUSE

Uniprot Description

Function: May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation. Ref.1 Ref.2

Subunit structure: Monomer.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart. Ref.7

Developmental stage: Expression is elevated in liver after birth but starts to decline around postnatal day 16. Ref.7

Post-translational modification: N- and O-glycosylated. Ref.6

Sequence similarities: Contains 6 EGF-like domains.

Research Articles on Dlk1

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Product Notes

The Dlk1 dlk1 (Catalog #AAA646035) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DLK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. Researchers should empirically determine the suitability of the Dlk1 dlk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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