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Flow Cytometry (FC/FACS) (FACS: DLK1 transfected HEK 293 cells were stained significantly using MBS6004123.)

Rat DLK1 Polyclonal Antibody | anti-Dlk1 antibody

DLK1 (Protein Delta Homolog 1, Fetal Antigen 1, Preadipocyte Factor 1, Pref-1, FA1)

Gene Names
Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
DLK1; Polyclonal Antibody; DLK1 (Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1); Anti -DLK1 (Protein Delta Homolog 1; anti-Dlk1 antibody
Ordering
For Research Use Only!
Host
Rat
Clonality
Polyclonal
Specificity
Recognizes human DLK1. Detects a band of ~42kD by Western blot.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Applicable Applications for anti-Dlk1 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Flow Cytometry, Western Blot
Immunogen
Recombinant human DLK1 (extracellular domain).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(FACS: DLK1 transfected HEK 293 cells were stained significantly using MBS6004123.)

Flow Cytometry (FC/FACS) (FACS: DLK1 transfected HEK 293 cells were stained significantly using MBS6004123.)

Western Blot (WB)

(Western blot analysis of human DLK1 using MBS6004123 at 1: 2000 dilution. A. 1. Human DLK1 (Fc protein). 2. Transfected human DLK1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate. B. 1. Human DLK-1 (Fc protein), 200ng. 2. Human DLK-1(ecd) (DYKDDDDK-tagged), 200ng. 3. Human DLL1(ecd) (DYKDDDDK-tagged), 200ng. 4. Mouse Vaspin (Fc protein) (control), 200ng. 5. Human Klotho (Fc protein) (control), 200ng. 6. Human Jagged1 (Fc protein) (control), 200ng.)

Western Blot (WB) (Western blot analysis of human DLK1 using MBS6004123 at 1: 2000 dilution. A. 1. Human DLK1 (Fc protein). 2. Transfected human DLK1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate. B. 1. Human DLK-1 (Fc protein), 200ng. 2. Human DLK-1(ecd) (DYKDDDDK-tagged), 200ng. 3. Human DLL1(ecd) (DYKDDDDK-tagged), 200ng. 4. Mouse Vaspin (Fc protein) (control), 200ng. 5. Human Klotho (Fc protein) (control), 200ng. 6. Human Jagged1 (Fc protein) (control), 200ng.)
Related Product Information for anti-Dlk1 antibody
DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.
Product Categories/Family for anti-Dlk1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,320 Da
NCBI Official Full Name
Dlk1
NCBI Official Synonym Full Names
delta-like 1 homolog (Drosophila)
NCBI Official Symbol
Dlk1
NCBI Official Synonym Symbols
FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
NCBI Protein Information
protein delta homolog 1; fetal antigen 1; preadipocyte factor 1; stromal cell derived protein 1; adipocyte differentiation inhibitor protein
UniProt Protein Name
Protein delta homolog 1
Protein Family
UniProt Gene Name
Dlk1
UniProt Synonym Gene Names
Dlk; Pref1; Scp-1; DLK-1; Pref-1; FA1
UniProt Entry Name
DLK1_MOUSE

Uniprot Description

DLK1: May have a role in neuroendocrine differentiation. Monomer. Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: calcium ion binding

Biological Process: embryonic development; regulation of gene expression; embryonic skeletal development; negative regulation of Notch signaling pathway; cell differentiation; post-embryonic development

Research Articles on Dlk1

Similar Products

Product Notes

The Dlk1 dlk1 (Catalog #AAA6004123) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DLK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Flow Cytometry, Western Blot. Researchers should empirically determine the suitability of the Dlk1 dlk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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