Rabbit Caspase-9, active, cleaved Polyclonal Antibody

Caspase-9, active, cleaved (CASP-9, Apoptotic Protease Mch-6, Apoptotic Protease-activating Factor 3, APAF-3, ICE-like Apoptotic Protease 6, ICE-LAP6, CASP9, MCH6)

Cat. Num. AAA644637

• Reactivity: Canine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: Caspase-9; active; cleaved; Polyclonal Antibody; cleaved (CASP-9; Apoptotic Protease Mch-6; Apoptotic Protease-activating Factor 3; APAF-3; ICE-like Apoptotic Protease 6; ICE-LAP6; CASP9; MCH6); Anti -Caspase-9; anti-Caspase-9; cleaved antibody

• Host: Rabbit
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human active, cleaved Caspase-9. May recognize the proform of caspase-9. On Western Blot, the proform is detected at ~50-60kD, the large subunit at ~35kD, and the small subunit at ~15kD. Intermediate cleavage forms may also be observed at ~21kD. Species Crossreactivity: canine, gerbil, mouse and rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-Caspase-9, active, cleaved antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (Paraffin): 1:1000-1:5000. Can also be used with frozen sections.; Immunoprecipitation: 1:50-1:200
• Immunogen: Recombinant corresponding to catalytically active human Caspase-9 protein.
• Positive Control: Cells or tissues undergoing apoptosis, lymphatic tissues, ischemic brain.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded canine brain sections using MBS644637 at 1:2000 (A and B). The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human brain sections stained for Active/Cleaved Caspase-9 expression using MBS644637 at 1:2000. A. Section from a patient 24 hr after head trauma. B. Control: section from a patient with no known neurological disease or head injury. Hematoxylin-eosin counterstain.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded canine brain sections stained for Active/Cleaved Caspase-9 expression using MBS644637 at 1:2000. A. Section from a canine brain 2 hr after reperfusion injury. B. Section from a dog brain sham control (brain surgery but no injury). Hematoxylin-eosin counterstain.)

Related Product Information for anti-Caspase-9, active, cleaved antibody

Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. They are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (2, 8, 9). Group II: effector caspases (3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). They are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes.

Product Categories/Family for anti-Caspase-9, active, cleaved antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 407280269
• NCBI Official Full Name: Chain F, Structure Of Cleaved, Card Domain Deleted Caspase-9