Carbonic Anhydrase IV Recombinant Protein | CA4 recombinant protein

Carbonic Anhydrase IV Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120292

• Gene Names: CA4; CAIV; Car4; RP17
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase IV; Carbonic Anhydrase IV Protein; Human; Recombinant (His Tag); Human Carbonic Anhydrase IV/CA4 Protein (His Tag); CAIV Protein; Car4 Protein; RP17 Protein; carbonic anhydrase IV; CA4 recombinant protein

• Host: CHO Stable Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys283

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CA4 (NP_000708.1)(Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA4 recombinant protein

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO₂ hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3^- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

References

Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1): 1-20.Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.

NCBI and Uniprot Product Information

• NCBI GI #: 4502519
• NCBI GeneID: 762
• NCBI Accession #: NP_000708.1
• NCBI GenBank Nucleotide #: NM_000717.4
• UniProt Accession #: P22748; Q6FHI7; B4DQA4
• Molecular Weight: 11,599 Da
• NCBI Official Full Name: carbonic anhydrase 4 preproprotein
• NCBI Official Synonym Full Names: carbonic anhydrase 4
• NCBI Official Symbol: CA4
• NCBI Official Synonym Symbols: CAIV; Car4; RP17
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA4
• UniProt Synonym Gene Names: CA-IV
• UniProt Entry Name: CAH4_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]

Uniprot Description

CA4: Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. Defects in CA4 are the cause of retinitis pigmentosa type 17 (RP17). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP17 inheritance is autosomal dominant. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; Membrane protein, GPI anchor; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 17q23

Cellular Component: Golgi apparatus; cell surface; sarcoplasmic reticulum; rough endoplasmic reticulum; basolateral plasma membrane; brush border membrane; secretory granule membrane; ER-Golgi intermediate compartment; trans-Golgi network; anchored to external side of plasma membrane; membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; sarcolemma

Molecular Function: carbonate dehydratase activity; protein binding; zinc ion binding

Biological Process: response to drug; response to steroid hormone stimulus; bicarbonate transport; organ development; one-carbon compound metabolic process

Disease: Retinitis Pigmentosa 17