Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Forskolin untreated (Lane 1 and Lane 3) and treated (Lane 2 and Lane 4) PC12 cell lysates were probed with Anti-phospho PKA-RIIalpha (Ser96) (1ug/ml). Phospho specifity was determined by Lambda-phosphatase treatment of lysates in lanes 3 and 4. All proteins were visualized using a Donkey-Anti-Rabbit IgG secondary antibody conjugated to HRPand detected using a chemiluminescence detection system Arrow indicates PKA-RIIalpha (Ser96) (~51kD).)
Rabbit Prkar2a Polyclonal Antibody | anti-Prkar2a antibody
Prkar2a, Phosphorylated (Ser96) (cAMP-dependent Protein Kinase Type II-alpha Regulatory Subunit) (HRP)
Gene Names
Prkar2a; AI317181; AI836829; RII(alpha); 1110061A24Rik
Reactivity
Mouse, Human, Rat
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Prkar2a; Polyclonal Antibody; Phosphorylated (Ser96) (cAMP-dependent Protein Kinase Type II-alpha Regulatory Subunit) (HRP); anti-Prkar2a antibody
Host
Rabbit
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Prkar2a, when phosphorylated at Ser96. Species Crossreactivity: human and rat.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
402
Applicable Applications for anti-Prkar2a antibody
Western Blot (WB)
Application Notes
WB: 1ug/ml
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Linear peptide corresponding to mouse Prkar2a, phosphorylated at Ser96, conjugated to KLH.
Conjugate
HRP
Control
Forskolin untreated and treated PC12 cell lysates
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Forskolin untreated (Lane 1 and Lane 3) and treated (Lane 2 and Lane 4) PC12 cell lysates were probed with Anti-phospho PKA-RIIalpha (Ser96) (1ug/ml). Phospho specifity was determined by Lambda-phosphatase treatment of lysates in lanes 3 and 4. All proteins were visualized using a Donkey-Anti-Rabbit IgG secondary antibody conjugated to HRPand detected using a chemiluminescence detection system Arrow indicates PKA-RIIalpha (Ser96) (~51kD).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Forskolin untreated (Lane 1 and Lane 3) and treated (Lane 2 and Lane 4) PC12 cell lysates were probed with Anti-phospho PKA-RIIalpha (Ser96) (1ug/ml). Phospho specifity was determined by Lambda-phosphatase treatment of lysates in lanes 3 and 4. All proteins were visualized using a Donkey-Anti-Rabbit IgG secondary antibody conjugated to HRPand detected using a chemiluminescence detection system Arrow indicates PKA-RIIalpha (Ser96) (~51kD).)
Product Categories/Family for anti-Prkar2a antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51
NCBI Official Full Name
cAMP-dependent protein kinase type II-alpha regulatory subunit
NCBI Official Synonym Full Names
protein kinase, cAMP dependent regulatory, type II alpha
NCBI Official Symbol
Prkar2a
NCBI Official Synonym Symbols
AI317181; AI836829; RII(alpha); 1110061A24Rik
NCBI Protein Information
cAMP-dependent protein kinase type II-alpha regulatory subunit
UniProt Protein Name
cAMP-dependent protein kinase type II-alpha regulatory subunit
Protein Family
UniProt Gene Name
Prkar2a
UniProt Entry Name
KAP2_MOUSE
Research Articles on Prkar2a
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Product Notes
The Prkar2a prkar2a (Catalog #AAA6444769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Prkar2a, Phosphorylated (Ser96) (cAMP-dependent Protein Kinase Type II-alpha Regulatory Subunit) (HRP) reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Prkar2a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Prkar2a prkar2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prkar2a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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