Rabbit anti-Human, Mouse PRKAR2B Polyclonal Antibody | anti-PRKAR2B antibody
PRKAR2B (cAMP-dependent Protein Kinase Type II-beta Regulatory Subunit) (MaxLight 550)
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Human brain tissue lysate was probed with Anti-PKA RII Beta (0.5ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG seconary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates PKA RII Beta (~46kD).)
NCBI and Uniprot Product Information
NCBI Description
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq, Jul 2008]
Uniprot Description
PKAR2B: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Protein type: Mitochondrial; Protein kinase, regulatory subunit
Chromosomal Location of Human Ortholog: 7q22
Cellular Component: centrosome; cytoplasm; dendritic spine; plasma membrane; dendritic shaft; cytosol; cAMP-dependent protein kinase complex
Molecular Function: ubiquitin protein ligase binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; organelle organization and biogenesis; activation of protein kinase A; learning; fatty acid metabolic process; signal transduction; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; G2/M transition of mitotic cell cycle; transmembrane transport; regulation of insulin secretion
