Rat anti-Mouse CDNF Monoclonal Antibody | anti-CDNF antibody

CDNF (Cerebral Dopamine Neurotrophic Factor, ARMET-like Protein 1, Conserved Dopamine Neurotrophic Factor, ARMETL1)

Cat. Num. AAA643306

• Gene Names: CDNF; ARMETL1
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: CDNF; Monoclonal Antibody; CDNF (Cerebral Dopamine Neurotrophic Factor; ARMET-like Protein 1; Conserved Dopamine Neurotrophic Factor; ARMETL1); Anti -CDNF (Cerebral Dopamine Neurotrophic Factor; anti-CDNF antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone Number: 12K60
• Specificity: Recognizes mouse CDNF. Species sequence homology: recombinant human CDNF (<5%)
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-CDNF antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry: 8-25ug/ml
• Immunogen: Recombinant corresponding to aa27-187 from mouse CDNF expressed in NSO cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(CDNF in Mouse Brain. CDNF was detected in perfusion fixed frozen sections of mouse brain using Rat Mouse CDNF Monoclonal Antibody at 25ug/ml overnight at 4°C. Tissue was stained using the NorthernLightsTM 557-conjugated Rat IgG Secondary Antibody and counterstained with DAPI (blue). Specific staining was localized to neuronal processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.)

Related Product Information for anti-CDNF antibody

CDNF, also called Armetl1, is a 17-19kD secreted protein that shares 52% aa identity with mouse MANF also called Armet. The Armet designation is not preferred, because the proteins as translated are not actually arginine-rich. However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfide bonds, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF). The mouse CDNF cDNA encodes a 187aa protein with a 24aa signal sequence and a 163 mature sequence. Mature mouse CDNF shares 80%, 87%, 83% and 82% aa identity with human, rat, equine and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre and postnatal mouse brain, they are mostly abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve. Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro. In a rat Parkinson's disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo.

Product Categories/Family for anti-CDNF antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 82880674
• NCBI GeneID: 441549
• NCBI Accession #: NP_001025125.2
• NCBI GenBank Nucleotide #: NM_001029954.2
• UniProt Accession #: Q49AH0; A2RUU0; B4DVW3
• Molecular Weight: 20,964 Da
• NCBI Official Full Name: cerebral dopamine neurotrophic factor
• NCBI Official Synonym Full Names: cerebral dopamine neurotrophic factor
• NCBI Official Symbol: CDNF
• NCBI Official Synonym Symbols: ARMETL1
• NCBI Protein Information: cerebral dopamine neurotrophic factor; ARMET-like protein 1; conserved dopamine neurotrophic factor; arginine-rich, mutated in early stage tumors-like 1
• UniProt Protein Name: Cerebral dopamine neurotrophic factor
• Protein Family: Cerebral dopamine neurotrophic factor
• UniProt Gene Name: CDNF
• UniProt Synonym Gene Names: ARMETL1
• UniProt Entry Name: CDNF_HUMAN

Uniprot Description

ARMETL1: Trophic factor for dopamine neurons. Prevents the 6- hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra. Belongs to the ARMET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: extracellular region

Molecular Function: growth factor activity