Rat anti-Mouse TREML2 Monoclonal Antibody | anti-TREML2 antibody

TREML2 (Trem-like Transcript 2 Protein, TLT-2, Triggering Receptor Expressed on Myeloid Cells-like Protein 2, Tlt2)

Cat. Num. AAA645833

• Gene Names: TREML2; TLT2; TLT-2; C6orf76; dJ238O23.1
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: TREML2; Monoclonal Antibody; TREML2 (Trem-like Transcript 2 Protein; TLT-2; Triggering Receptor Expressed on Myeloid Cells-like Protein 2; Tlt2); Anti -TREML2 (Trem-like Transcript 2 Protein; anti-TREML2 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone Number: 12K189
• Specificity: Recognizes mouse TREML2. Does not react with recombinant mouse TREM-1,-2,-2b, -3, -4, rmTREML1/TLT-1, rmTREML4/TLT-4, or recombinant human NKp44.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-TREML2 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 2.5ug labels 10e6 cells
• Immunogen: Recombinant corresponding to aa25-270 from mouse TREML2 expressed in CHO-S cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Flow Cytometry (FC/FACS)

(Detection of TREML2 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Rat Mouse TREML2/TLT-2 Monoclonal Antibody or isotype control antibody followed by Phycoerythrin-conjugated Rat IgG Secondary Antibody.)

Related Product Information for anti-TREML2 antibody

TREML2, also called TLT-2 is a 33kD type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329aa precursor that contains a 24aa signal sequence, a 246aa extracellular domain (ECD), a 21aa transmembrane segment, and a 38aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain aa25-126 and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49% aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines. TLT-2 is also expressed constitutively on CD8+ T cells.

Product Categories/Family for anti-TREML2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 170932501
• NCBI GeneID: 79865
• NCBI Accession #: NP_079083.2
• NCBI GenBank Nucleotide #: NM_024807.3
• UniProt Accession #: Q5T2D2; Q08AP8; Q08AP9; Q8IWY0; Q9H8E9
• Molecular Weight: 35,127 Da
• NCBI Official Full Name: trem-like transcript 2 protein
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells-like 2
• NCBI Official Symbol: TREML2
• NCBI Official Synonym Symbols: TLT2; TLT-2; C6orf76; dJ238O23.1
• NCBI Protein Information: trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like protein 2
• UniProt Protein Name: Trem-like transcript 2 protein
• Protein Family: Trem-like transcript 2 protein
• UniProt Gene Name: TREML2
• UniProt Synonym Gene Names: C6orf76; TLT2; TLT-2
• UniProt Entry Name: TRML2_HUMAN

NCBI Description

TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties (Allcock et al., 2003 [PubMed 12645956]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation. Ref.1 Ref.6

Subunit structure: Interacts with CD276 and this interaction enhances T-cell activation. Ref.6

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Detected in cultured B-cells, T-cell leukemia and monocyte leukemia. Expressed constitutively on CD8 T-cells and induced on CD4 T-cells after activation. Ref.1 Ref.6

Induction: Induced in CD4 T-cells by concanavalin-A. Ref.6

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution: The sequence AAI25079.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI25080.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14668.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI19858.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI19909.1 differs from that shown. Reason: Erroneous initiation.