Rabbit anti-Human MSH3 Polyclonal Antibody | anti-MSH3 antibody

MSH3 (MutS Homolog 3, DUP, MRP1) (HRP)

Cat. Num. AAA6427479

• Gene Names: MSH3; DUP; MRP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MSH3; Polyclonal Antibody; MSH3 (MutS Homolog 3; DUP; MRP1) (HRP); anti-MSH3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MSH3.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MSH3 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human MSH3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MSH3 antibody.)

Product Categories/Family for anti-MSH3 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 284813531
• NCBI GeneID: 4437
• NCBI Accession #: NP_002430.3
• NCBI GenBank Nucleotide #: NM_002439.4
• UniProt Accession #: P20585; Q6PJT5; Q86UQ6; Q92867; A1L482; A6NMM6
• Molecular Weight: 127,412 Da
• NCBI Official Full Name: DNA mismatch repair protein Msh3
• NCBI Official Synonym Full Names: mutS homolog 3 (E. coli)
• NCBI Official Symbol: MSH3
• NCBI Official Synonym Symbols: DUP; MRP1
• NCBI Protein Information: DNA mismatch repair protein Msh3; hMSH3; mismatch repair protein 1; divergent upstream protein
• UniProt Protein Name: DNA mismatch repair protein Msh3
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH3
• UniProt Synonym Gene Names: DUC1; DUG; hMSH3; DUP; MRP1
• UniProt Entry Name: MSH3_HUMAN

NCBI Description

The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. [provided by RefSeq, Mar 2011]

Uniprot Description

MSH3: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion- deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC). Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: nucleoplasm; nuclear chromosome; membrane; nucleus; MutSbeta complex

Molecular Function: DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; protein homodimerization activity; dinucleotide repeat insertion binding; oxidized purine DNA binding; mismatched DNA binding; loop DNA binding; Y-form DNA binding; centromeric DNA binding; protein binding; enzyme binding; single guanine insertion binding; guanine/thymine mispair binding; dinucleotide insertion or deletion binding; ATP binding; single-stranded DNA binding

Biological Process: positive regulation of helicase activity; negative regulation of DNA recombination; mismatch repair; meiotic recombination; somatic recombination of immunoglobulin gene segments; DNA repair; maintenance of DNA repeat elements; meiotic mismatch repair

Disease: Endometrial Cancer