Rabbit Smg1 Polyclonal Antibody | anti-SMG1 antibody

Smg1 Antibody

Cat. Num. AAA9612387

• Gene Names: SMG1; ATX; LIP; 61E3.4
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Smg1; Polyclonal Antibody; Smg1 Antibody; 61E3.4; ATX; hSMG-1; hSMG1; KIAA0421; Lambda interacting protein; Lambda-interacting protein; Lambda/iota protein kinase C interacting protein; Lambda/iota protein kinase C-interacting protein; LIP; Phosphatidylinositol 3 kinase related kinase; Phosphatidylinositol 3 kinase related protein kinase; PI 3 kinase related kinase SMG 1; Serine/threonine protein kinase SMG1; Serine/threonine-protein kinase SMG1; SMG-1; smg1; SMG1_HUMAN; anti-SMG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Smg1 Antibody detects endogenous levels of total Smg1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 3661

• Applicable Applications for anti-SMG1 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Smg1, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using Smg1 Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612387 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612387 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612387 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612387 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612387) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-SMG1 antibody

Function: Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.

Post Translational Modifications: Autophosphorylated.

Subcellular Location: Nucleus. Cytoplasm.

Tissue Specificity: Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.

Subunit Structure: Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2. Interacts with UPF2.

Similarity: Belongs to the PI3/PI4-kinase family.

NCBI and Uniprot Product Information

• NCBI GI #: 62243658
• NCBI GeneID: 23049
• NCBI Accession #: NP_055907.3
• NCBI GenBank Nucleotide #: NP_055907.3
• UniProt Accession #: Q96Q15
• Molecular Weight: Observed Molecular Weight: (Observed)410kD.; Predicted Molecular Weight: (Calculated)411kDa.
• NCBI Official Full Name: serine/threonine-protein kinase SMG1
• NCBI Official Synonym Full Names: SMG1 nonsense mediated mRNA decay associated PI3K related kinase
• NCBI Official Symbol: SMG1
• NCBI Official Synonym Symbols: ATX; LIP; 61E3.4
• NCBI Protein Information: serine/threonine-protein kinase SMG1
• UniProt Protein Name: Serine/threonine-protein kinase SMG1
• Protein Family: Probable serine/threonine-protein kinase
• UniProt Gene Name: SMG1
• UniProt Synonym Gene Names: ATX; KIAA0421; LIP; SMG-1; hSMG-1
• UniProt Entry Name: SMG1_HUMAN

NCBI Description

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]